SHEEP REQUEST FOR FLUORESCENT MICROSCOPE

The requested instrument is an All-in-One fluorescence microscope (model #BZ-X700)manufactured by Keyence. This microscope allows high-resolution and high-throughputimaging and analysis while retaining user-friendly operation. It features expandedcapacities and includes a high sensitivity cooled monochrome camera, a largemotorized stage, high-speed autofocus and low photobleach and real-time imageoverlay modes. It also promotes rapid data analysis and includes image stitching,optical sectioning and 3-dimensional modeling functions, as well as the ability to monitorfluorescently labeled protein movement within in the cell in real time or with time lapse.The acquisition of multiple filters also allows imaging of several fluorescence labelsessentially simultaneously such that multiple parameters can be monitored.The following research projects will be supported by this instrument:Dr. Wendy Bollag, VA Merit CX001357: Epidermal Aquaporin-3 in PsoriasisDr. Adviye Ergul, VA Merit BX000347: Cerebral Arteriole Structure/Function in DiabeticIschemic Brain InjuryDr. Tohru Fukai, VA Merit BX001232: Role of Copper Transporters in AtherosclerosisDr. William Hill, VA Merit CX000930: Bad to the Bone: Age-related Rise in Serum SDF-1 Leads to Bone Loss with AgeDr. Almira Vazdarjanova, VA Merit BX001978: Impaired Hippocampal Function as aRisk Factor for Post-Traumatic Stress Disorder