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A convenient method for the preparation of asialo-GM1

  • Noriyuki Kasai(corresponding author)
    ,
  • Laurel O. Sillerud
    ,
  • Robert K. Yu
*Corresponding author for this work
  • Yale University
Scholary Output:
Contribution to journal
Article
 Peer-review

Abstract

A convenient and efficient procedure has been devised for the large-scale preparation of asialo-GM1 from bovine brain gangliosides. The procedure relies on the complete desialylation of brain ganglio-sides, consisting primarily of GM1, GD1a, GD1b and GT1b, by mild formic acid hydrolysis (0.1 N, 100 C; 2 hr). Following the hydrolysis step, asialo-GM1 can be isolated and purified by Folch partitioning and Iatrobeads column chromatography, with an overall yield of more than 50%.

Publication Information

Output type

Scholary Output:
Contribution to journal
Article
 Peer-review

Original language

English (US)

Pages from-to (Number of pages)

Pages 107-110 (4 pages)

Journal (Volume, Issue Number)

Lipids (Volume 17, Issue 2)

Publication milestones

  • Published - 02/1982

Publication status

Published - 02/1982

ISSN

0024-4201

Publication IDs

  • Scopus: 0020093397
  • PubMed: 7087683

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Scopus
citations
45, opens in new tab
Fractional count
1
Fractional count
0.33
Fractional count
2
Fractional count
0.67
Fractional count
1
Fractional count
1

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Citation count
45
Captures
6

Funding Details

FunderFunding number
NINDS
R01NS011853