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A highly sensitive microbioassay for the molluscan neuropeptide FMRFamide

  • Gregg T. Nagle(corresponding author)
    ,
  • Michael J. Greenberg
*Corresponding author for this work
  • Florida State University
Scholary Output:
Contribution to journal
Article
 Peer-review

Open access

Abstract

1. An organ bath of very small volume (100 μl) has been designed for the superfusion of long thin molluscan muscles. The plan of this microbath is presented in detail. 2. Isolated in the microbath, radula protractor muscles from small specimens of the lightning whelk (Busycon contrarium) can be used to assay as little as 0.1 pmols of the molluscan neuropeptide FMRFamide.

Publication Information

Output type

Scholary Output:
Contribution to journal
Article
 Peer-review

Original language

English (US)

Pages from-to (Number of pages)

Pages 101-105 (5 pages)

Journal (Volume, Issue Number)

Comparative Biochemistry and Physiology. Part C, Comparative (Volume 71, Issue 1)

Publication milestones

  • Published - 1982

Publication status

Published - 1982

ISSN

0306-4492

Publication IDs

  • Scopus: 0020041429

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Funding Details

Acknow/edyements This work was supported by NIH Grant HL-09283 to M.J.G. and is Contribution No. 163 from the Tallahassee, Sopchoppy & Gulf Coast Marine Biological Association. We thank James Hogg and John Highsmith for constructing the microbath, Nancy Hopkins for the mechanical drawing and Dr John A. Watts for the microbath photograph. Dr David Price provided helpful suggestions in the original design.