A micropreparation of fluorescein conjugates of immunoglobulin G and fab from serum
- Joy G. Mohanty,
- Ken S. Rosenthal(corresponding author)
- Northeastern Ohio Universities College of Medicine
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Article
Peer-reviewAbstract
Protein A-Sepharose CL-4B (PAS) was used to isolate rabbit immunoglobulin G from crude anti-herpes simplex virus-1 serum. Papain treatment of the PAS-bound immunoglobulin G released Fab fragments from the solid support, while Fc-containing fragments remained bound to PAS. PAS-immobilized immunoglobulin G was fluoresceinated by reaction with fluorescein isothiocyanate followed by papain cleavage to yield fluorescein-conjugated Fab fragments in solution. These fragments retained activity toward herpes simplex virus-1 infected Vero cells as evaluated by immunofluorescence. This novel procedure represents the fastest and simplest method for preparing Fab or fluoresceinated Fab fragments directly from any volume of immune serum.
Publication Information
Output type
Scholary Output:
Contribution to journal
Article
Peer-reviewOriginal language
English (US)Pages from-to (Number of pages)
Pages 361-365 (5 pages)Journal (Volume, Issue Number)
Analytical Biochemistry (Volume 146, Issue 2)Publication milestones
- Published - 05/01/1985
Publication status
Published - 05/01/1985
ISSN
0003-2697Publication IDs
- Scopus: 0022058714
- PubMed: 3927771
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Funding Details
We thank Cheryl Hodnichak and Kim Straitiff for their technical assistance. This work was supported in part by an American Cancer Society Pilot Research Grant from the Ohio Division to K.S.R.
FundersFunding numbers
Division of Periodontics, Ohio State University
-ACS
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