Accumulation of A Globo-series Glycolipid Having Galα 1–3 Gal In Pc12h Pheochromocytoma Cells
- Toshio Ariga(corresponding author),
- Robert K. Yu,
- J. Neel Scarsdale,
- Minoru Suzuki,
- Yoichiro Kuroda,
- Hisayo Kitagawa
- Tokyo Metropolitan Institute of Medical Science,
- Yale University,
- Niigata University
Abstract
In a previous paper, we reported the presence of globoside as a major neutral glycolipid in PC 12 pheochromocytoma cells [Ariga, T., Macala, L. J., Saito, M., Margolis, R. K., Greene, L. A., Margolis, R. U., & Yu, R. K. (1988) Biochemistry 27, 52–58], Recently, we found that subcloned PC12h cells accumulated another unusual neutral glycolipid. In order to characterize this glycolipid, PC12h cells were subcutaneously transplanted into rats. The induced tumor tissue accumulated two major neutral glycolipids, which were purified by Iatrobeads column and preparative thin-layer chromatographies. One of the glycolipids was found to be globoside, and the other had a globotriaosyl structure with an additional terminal Galα 1–3 residue. Its structure was determined by fast atom bombardment mass spectrometry, two-dimensional proton nuclear magnetic resonance spectrometry (2D NMR), permethylation study, sequential degradation with exoglycosidase, and mild acid hydrolysis to be Gal(αl-3)Gal(αl-4)Gal(β1-4)Glc(β1-1′)Cer.
Publication Information
Output type
Original language
English (US)Pages from-to (Number of pages)
Pages 5335-5340 (6 pages)Journal (Volume, Issue Number)
Biochemistry (Volume 27, Issue 14)Publication milestones
- Published - 07/01/1988
Publication status
ISSN
0006-2960Publication IDs
- Scopus: 0023726401
- PubMed: 3167050