Ceramide-induced vasorelaxation: An inhibitory action on protein kinase C
- Douglas G. Johns(corresponding author),
- Jong Shiaw Jin,
- Dixon W. Wilde,
- R. Clinton Webb
- University of Michigan, Ann Arbor,
- National Defense Medical University
Open access
Abstract
Experiments were designed to examine the role of sphingosine, PP2A phosphatases, and protein kinase C (PKC) inhibition in mediating the vasodilatory effects of ceramide in rat thoracic aorta. Sphingosine did not cause vasorelaxation, and oleoylethanolamine, a ceramidase inhibitor, did not affect sphingomyelinase-induced relaxation. Okadaic acid potentiated the relaxation response to ceramide. These observations rule out involvement of sphingosine and PP2A phosphatases in mediating ceramide-induced relaxation. Sphingomyelinase attenuated contractile and single-cell intracellular calcium responses to phorbol ester. Chelerythrine incubation potentiated the relaxation response to ceramide. These observations support a role for PKC inhibition in mediating the vasodilatory effects of ceramide. Copyright (C) 1999 Elsevier Science Inc.
Publication Information
Output type
Original language
English (US)Pages from-to (Number of pages)
Pages 415-421 (7 pages)Journal (Volume, Issue Number)
General Pharmacology: The Vascular System (Volume 33, Issue 5)Publication milestones
- Published - 11/1999
Publication status
ISSN
0306-3623Publication IDs
- Scopus: 0032726839
- PubMed: 10553883