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DNA from various tissues of far eastern larches and its applicability for RAPD assay

  • I. Yu Sazonova
    ,
  • M. M. Kozyrenko
    ,
  • E. V. Artyukova
    ,
  • G. D. Reunova
    ,
  • Y. N. Zhuravlev
Scholary Output:
Contribution to journal
Article
 Peer-review

Open access

Abstract

We studied applicability of RAPD assay for investigating genetic variability of far eastern larches. Techniques to isolate DNA from larch vegetative organs (needles, young shoots and stems) and megagametophytes were selected, and conditions of polymerase chain reaction were optimized. Fifty out of 84 random primers proved efficient, and 22 and 12 primers initiated weak and no synthesis, respectively. The tested primers worked efficiently on the DNA isolated from both diploid and haploid tissues.

Publication Information

Output type

Scholary Output:
Contribution to journal
Article
 Peer-review

Original language

English (US)

Pages from-to (Number of pages)

Pages 196-201 (6 pages)

Journal (Volume, Issue Number)

Biology Bulletin (Volume 28, Issue 2)

Publication milestones

  • Published - 2001

Publication status

Published - 2001

ISSN

1062-3590

Publication IDs

  • Scopus: 0034858650

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Publication metrics

Metrics

Scopus
citations
1, opens in new tab
Fractional count
1
Fractional count
0.20
Fractional count
4
Fractional count
0.80
Fractional count
1
Fractional count
1
SciVal
Author count
5
SciVal
citations
1
SciVal
Paper percentile
33

Funding Details

ACKNOWLEDGMENTS This work was supported by the Biotic Service and Inventories Program of the National Science Foundation of USA (project Dep-9505031, Theodor W. Pietsch, principal investigator) and State Research Program Biodiversity.