DNA from various tissues of the Far Eastern larch and its applicability for RAPD assay
Original title: DNK iz raznykh organov dal'nevostochnykh listvennits i ee prigodnost' dlia RAPD-analiza.
- Irina Yurievna Sazonova(corresponding author),
- M. M. Kozyrenko,
- E. V. Artiukova,
- G. D. Reunova,
- I. N. Zhuravlev
- ,
- Russian Academy of Sciences
Scholary Output:
Contribution to journal
Article
Peer-reviewAbstract
We studied applicability of RAPD assay for investigating genetic variability of far eastern larches. Techniques to isolate DNA from larch vegetative organs (needles, young shoots and stems) and megagametophytes were selected, and conditions of polymerase chain reaction were optimized. Fifty out of 84 random primers proved efficient, and 22 and 12 primers initiated weak and no synthesis, respectively. The tested primers worked efficiently on the DNA isolated from both diploid and haploid tissues.
Publication Information
Output type
Scholary Output:
Contribution to journal
Article
Peer-reviewOriginal language
RussianPages from-to (Number of pages)
Pages 243-248 (6 pages)Journal (Volume, Issue Number)
Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiiskaia akademiia nauk (Issue 2)Publication milestones
- Published - 01/01/2001
Publication status
Published - 01/01/2001
ISSN
1026-3470Publication IDs
- Scopus: 0035291960
- PubMed: 11357392
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Publication metrics
Metrics
SciVal
citations
1
SciVal
FWCI
0.33
SciVal
Author count
5
SciVal
Paper percentile
33
Fractional count
1
Fractional count
0.20
Fractional count
4
Fractional count
0.80
Fractional count
1
Fractional count
1
PlumX
Captures
2
Citation count
1