Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro
- Zhenyang Zheng,
- Yan Leng,
- Chen Zhou,
- Zhenyu Ma,
- Zhigang Zhong,
- Sun Yat-Sen University,
Abstract
Matrix metalloproteinase-1 (MMP-1) is a member of the family of zinc-dependent endopeptidases that are capable of degrading extracellular matrix (ECM) and certain non-matrix proteins. It has been shown that MMP-1 can enhance muscle regeneration by improving the differentiation and migration of myoblasts. However, it is still not known whether MMP-1 can promote the myogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). To address this question, we isolated BMSCs from C57BL/6J mice and investigated the effects of MMP-1 on their proliferation and myogenic differentiation. Our results showed that MMP-1 treatment, which had no cytotoxic effects on BMSCs, increased the mRNA and protein levels of MyoD and desmin in a dose-dependent manner, indicating that MMP-1 promoted myogenic differentiation of BMSCs in vitro. These results suggest that BMSCs may have a therapeutic potential for treating muscular disorders.
Publication Information
Output type
Original language
English (US)Pages from-to (Number of pages)
Pages 309-314 (6 pages)Journal (Volume, Issue Number)
Biochemical and Biophysical Research Communications (Volume 428, Issue 2)Publication milestones
- Published - 11/16/2012
Publication status
ISSN
0006-291XPublication IDs
- Scopus: 84869204630
- PubMed: 23085232