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Liberation of hydroxyproline from gelatin by acid hydrolysis

  • David H. Pashley(corresponding author)
    ,
  • C. Keith Claycomb
    ,
  • Gordon W. Summers
*Corresponding author for this work
  • Oregon Health and Science University
Scholary Output:
Contribution to journal
Article
Peer-review

Abstract

A simple technique is described for the hydrolysis of gelatin resulting in good recovery of hydroxyproline. Hydrolysis of not more than 240 mg of gelatin in 4 ml of approximately 6 N HCl gives maximum yield of hydroxyproline after 12 hr at 100°C, with the required time decreasing to 1 hr at 130-150°.

Publication Information

Output type

Scholary Output:
Contribution to journal
Article
Peer-review

Original language

English (US)

Pages from-to (Number of pages)

Pages 154-156 (3 pages)

Journal (Volume, Issue Number)

Analytical Biochemistry (Volume 15, Issue 1)

Publication milestones

  • Published - 04/1966

Publication status

Published - 04/1966

ISSN

0003-2697

Publication IDs

  • Scopus: 0013896084
  • PubMed: 5959419

Publication metrics

Metrics

Scopus
citations
Fractional count
1
Fractional count
0.33
Fractional count
2
Fractional count
0.67
Fractional count
1
Fractional count
1

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Social media
6
Citation count
11
Captures
4

Funding Details

The authors gratefully acknowledge the assistance of S. Robert &how. This investigation was supported in part by the United States Public Health Service Research Grant DE 01908-02 from the National Institut~c of Dental Research, National Institutes of Health, Bethesda, Maryland.
FundersFunding number
National Institut~c of Dental Research
-
NIH
-
USPHS
DE 01908-02