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Modulation of soluble and membrane-bound TNF-induced phenotypic and functional changes of human brain microvascular endothelial cells by recombinant TNF binding protein I

  • J. Lou(corresponding author)
    ,
  • A. Ythier
    ,
  • D. Burger
    ,
  • L. Zheng
    ,
  • P. Juillard
    ,
  • Rudolf Lucas
*Corresponding author for this work
  • University of Geneva
    ,
  • Merck KGaA
Scholary Output:
Contribution to journal
Article
 Peer-review

Open access

Sustainable Development Goals

  • SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well

Abstract

In this study, the effects of TNF binding protein I (TBP I) on TNF- induced changes of human brain microvascular endothelial cells (MVEC) were investigated. TBP I completely abolished TNF-induced IL-6 production and E- selectin induction, while it partially inhibited TNF-induced IL-8 production and up-regulation of ICAM-I and VCAM-I. Moreover, TBP I significantly inhibited TNF-induced cytotoxicity and leukocyte adherence on human brain MVEC. The inhibitory activity of TBP I for TNF was dose-dependent and related to the time of administration after TNF stimulation. In addition, TBP I inhibited membrane-bound TNF induced activation of human brain MVEC, but the concentration required was about 10-fold higher than that for soluble TNF. These results indicate a therapeutic potential for TBP I in diseases of the central nervous system associated with TNF overproduction.

Publication Information

Output type

Scholary Output:
Contribution to journal
Article
 Peer-review

Original language

English (US)

Pages from-to (Number of pages)

Pages 107-115 (9 pages)

Journal (Volume, Issue Number)

Journal of Neuroimmunology (Volume 77, Issue 1)

Publication milestones

  • Published - 07/1997

Publication status

Published - 07/1997

ISSN

0165-5728

Publication IDs

  • Scopus: 0030876809
  • PubMed: 9209275

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Publication metrics

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SciVal
FWCI
0.61
SciVal
Author count
8
SciVal
citations
20
SciVal
Paper percentile
72
Scopus
citations
20, opens in new tab
Fractional count
1
Fractional count
0.13
Fractional count
7
Fractional count
0.88
Fractional count
1
Fractional count
1

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Captures
13
Citation count
20

Funding Details

This work was supported by grants from Ares-Serono, Geneva, Switzerland (G.E.G.), the Swiss Society for Multiple Sclerosis (to D.B. and G.E.G), the ARSEP, Paris (to G.E.G) and the Swiss National Science Foundation (to G.E.G. and J.-M.D). We thank Dr. D. Wallach (Rehovot), A.J.H. Gearing (Oxford) and D. Degroote (Fleurus) for their generous gift of antibodies and Dr. B. Chappuis (Transfusion Centre, Geneva University Hospital) for blood aliquots of normal human donors.