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Performance of a transcription-mediated-amplification HIV-1 RNA assay in pooled specimens

  • Nicola M. Zetola
    ,
  • Ann Mintie
    ,
  • Sally Liska
    ,
  • Ernest Wong
    ,
  • Joan Dunn Williams
    ,
  • Michael P. Busch
*Corresponding author for this work
  • University of California at San Francisco
    ,
  • San Francisco Department of Public Health
    ,
  • Vitalant
    ,
  • STD Prevention and Control Services
Scholary Output:
Contribution to journal
Article
 Peer-review

Sustainable Development Goals

  • SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well

Abstract

Background: Very limited data exist on the comparative performance of nucleic acid amplification tests (NATs) for the screening of pooled specimens for acute human immunodeficiency virus (HIV) infection. Study design: In this study, we compared a transcription-mediated-amplification assay (Procleix® HIV-1 Discriminatory Assay, [TMA]) with a branched DNA assay (Bayer Versant HIV-1 RNA 3.0 assay, [bDNA]). Results: After re-testing 1552 samples that were negative for HIV RNA by bDNA, we found one additional positive sample with the TMA assay. Conclusion: Our results suggest that TMA could potentially detect acute HIV infections missed by other technologies.

Publication Information

Output type

Scholary Output:
Contribution to journal
Article
 Peer-review

Original language

English (US)

Pages from-to (Number of pages)

Pages 68-70 (3 pages)

Journal (Volume, Issue Number)

Journal of Clinical Virology (Volume 40, Issue 1)

Publication milestones

  • Published - 09/2007

Publication status

Published - 09/2007

ISSN

1386-6532

Publication IDs

  • Scopus: 34548129582
  • PubMed: 17662648

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Funding Details

Financial Support: This project was supported in part by the Universitywide AIDS Research Program grant CH05-SMCHC-612 and the San Francisco Department of Public Health. Gen-Probe, Inc. supplied reagents for HIV Procleix HIV-1 Discriminatory RNA testing at no cost to the study.
FunderFunding number
Universitywide AIDS Research Program
CH05-SMCHC-612