Primary study of expression of matrix metalloproteinase-2 in yeast Pichia pastoris

Matrix metalloproteinase-2 (MMP-2) plays important role in tumor invasion and metastasis. MMP-2 is expressed in yeast expression system for further studying its function mechanism. The expression sequence of MMP-2 (gelatinase A) have been obtained by PCR amplifying, restriction enzyme cut and sequencing analysis demostrate that the sequence is correct. The expression vector pPIC9/GelA containing gelatinase A sequence is constructed and transformed the yeast Pichia pastoris. The gelatin enzymography demostrate that recombinant protein secreted by transformant yeast is capable of degrading gelatin and the molecular weight of the matrix metalloproteinase-2 is 66 ku in SDS-PAGE.