Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3
- Jean Denis Cornillot,
- Michel Pontet,
- Catherine Dupuy,
- ,
- Michel Caron,
- Raymonde Joubert-Caron
- Université Paris 13,
- Centre de Transfusion Sanguine des Armées,
- Institut national de la santé et de la recherche médicale,
- Hôpital Avicenne
Scholary Output:
Contribution to journal
Article
Peer-reviewAbstract
Antisera raised against galectin-1 exhibit crossreactivities with other galectins or related molecules. In order to overcome this problem, 3 monoclonal antibody to human brain galectin-1 was obtained by selecting clones without reactivity toward galectin-3. This mAb specifically bound galectin-1 of various animal origins but neither galectin-2 nor galectin-3. Western-blotting analysis of soluble human brain extracts after 2D gel electrophoresis revealed only the two most acidic isoforms of galectin-1. The ability of this mAb to bind galectin-1-asialofetuin complexes indicates that its epitope is not localized in galacarbohydrate recognition domain of galectin-1. This particularity induces with efficiency its monospecificity.
Publication Information
Output type
Scholary Output:
Contribution to journal
Article
Peer-reviewOriginal language
English (US)Pages from-to (Number of pages)
Pages 425-432 (8 pages)Journal (Volume, Issue Number)
Glycobiology (Volume 8, Issue 5)Publication milestones
- Published - 05/1998
Publication status
Published - 05/1998
ISSN
0959-6658Publication IDs
- Scopus: 0031798590
- PubMed: 9597540
Publication metrics
Metrics
SciVal
FWCI
0.11
SciVal
Author count
8
SciVal
citations
5
SciVal
Paper percentile
47
Fractional count
1
Fractional count
0.13
Fractional count
7
Fractional count
0.88
Fractional count
1
Fractional count
1
PlumX, opens in new tab
Captures
10
Citation count
7
Funding Details
This work has been supported by grants from the Ministère de l’Education Nationale de l’Enseignement Supérieur et de la Recherche. J. D. Cornillot is an Allocataire de Recherche (Ministère de l’Education Nationale de l’Enseignement Supérieur et de la Recherche). We thank Dr. F.-T. Liu (La Jolla Institute for Allergy and Immunology, San Diego, CA), Dr. A.-P. Sève (INSERM U 180, Paris, France), and Dr. M. A. Gitt and Dr. H. Leffler (Department of Psychiatry and Langley Porter Psychiatric Institute, University of California, San Francisco, CA) for the gift of human and mouse galectin-3 and galectin-2, respectively, and also Dr L. Astic (Laboratoire de Physiologie Neurosensorielle, Université Claude Bernard, Lyon-1) and Dr. M. Fèvre-Montange (Laboratoire de Neurobiologie Expérimentale et Physiopathologie, Faculté de Médecine R. Laennec, Université Lyon-1) for their assistance in immunochemistry and in situ hybridization, respectively.