Skip to search boxSkip to navigationSkip to main content

1H-2D-nuclear magnetic resonance applied to the primary structure determination of a novel octasaccharide glycolipid isolated from the spermatozoa of bivalves

  • J. Neel Scarsdale
    ,
  • James H. Prestegard(corresponding author)
    ,
  • Susumu Ando
    ,
  • Taro Hori
    ,
  • Robert K Yu
*Corresponding author for this work
  • Yale University
    ,
  • Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology
    ,
  • Shiga University
Scholary Output:
Contribution to journal
Article
 Peer-review

Open access

Abstract

High resolution, two-dimensional 1H-n.m.r. spectroscopy has been used to confirm a proposed primary structure of a glycolipid having an octasaccharide head-group. Pure absorption and relay experiments were found to be particularly useful in establishing connectivities in poorly resolved regions of the spectrum. The spectral assignments, which indicate novel linkages including an internal fucopyranosyl residue as well as terminal xylosyl and 4-O-methylglucopyranosyluronic acid groups, add to a growing data base for structural characterization through n.m.r. spectroscopy.

Publication Information

Output type

Scholary Output:
Contribution to journal
Article
 Peer-review

Original language

English (US)

Pages from-to (Number of pages)

Pages 45-56 (12 pages)

Journal (Volume, Issue Number)

Carbohydrate Research (Volume 155, Issue C)

Publication milestones

  • Published - 11/01/1986

Publication status

Published - 11/01/1986

ISSN

0008-6215

Publication IDs

  • Scopus: 0022819464
  • PubMed: 3791301

Access to documents

Publication metrics

Metrics

Scopus
citations
12, opens in new tab
Fractional count
1
Fractional count
0.20
Fractional count
4
Fractional count
0.80
Fractional count
1
Fractional count
1

PlumX, opens in new tab

Captures
3
Citation count
12

Funding Details

The authors thank Dr. Dennis Hare for the use of his n.m.r. processing software. The research benefited from instrumentationp rovided through shared instrumentationp rograms of the National Institute of General Medical Sciences (grant GM-32 243 Sl) and the Division of Research Resources of NIH (grant RR 02 379). ‘This work was supported by grants GM-33 225 and NS-11 853 from the National Institutes o?’Health, and by a predoctoral fellowship (to J.N.S.) from the National Science Foundation. ‘To whom correspondence should be addressed.
FundersFunding numbers
Laboratory of Biological Structure, National Institutes of Dental Research, National Institutes of Health
-
NSF
-
NIH
RR 02 379
NIGMS
R01GM032243, R01GM033225
NINDS
R01NS011853