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Surface-enhanced Raman scattering for cancer diagnostics: Detection of the BCL2 gene

*Corresponding author for this work
  • Oak Ridge National Laboratory
Scholary Output:
Contribution to journal
Article
 Peer-review

Sustainable Development Goals

  • SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well

Abstract

A method of detection for cancer genes, such as B-cell lymphoma 2 gene, has been developed using surface-enhanced Raman scattering-active substrates. This method uses Raman active dye-labeled DNA gene probes and metallic nanostructures as surface-enhanced Raman scattering-active platforms. A self-assembled monolayer system composed of mercapto hexane-labeled, single-strand DNA (SH-(CH2)6-ssDNA/ 6-mercapto-1-hexanol) is formed on a silver surface. The Raman-active dye-labeled gene is hybridized with its complementary probe, which is immobilized on the silver surface. The surface-enhanced Raman gene probes in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not involve the use of radioactive labels and have great potential to provide both sensitivity and selectivity. The utility of this approach is investigated.

Publication Information

Output type

Scholary Output:
Contribution to journal
Article
 Peer-review

Original language

English (US)

Pages from-to (Number of pages)

Pages 669-675 (7 pages)

Journal (Volume, Issue Number)

Expert Review of Molecular Diagnostics (Volume 3, Issue 5)

Publication milestones

  • Published - 09/2003

Publication status

Published - 09/2003

ISSN

1473-7159

Publication IDs

  • Scopus: 0041820140
  • PubMed: 14510186

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Funding Details

This work was sponsored by the US Department of Energy (DOE) Office of Biological and Environmental Research and the DOE Chemical and Biological Nonproliferation Program and under contract DE-AC05-00OR22725 with UT-Battelle, LLC. Mustafa Culha and David Stokes are supported by an appointment to Oak Ridge National Laboratory Postdoctoral Research Associates Program, administered jointly by the Oak Ridge National Laboratory and Oak Ridge Institute for Science and Education.