TY - JOUR
T1 - A method for the in vitro determination of feline corneal endothelial permeability
AU - Mcdermott, Mark L.
AU - Watsky, Mitchell A.
AU - Geroski, Dayle H.
AU - Edelhauser, Henry F.
N1 - Funding Information:
ACKNOWLEDGEMENTS Supported in part by an unrestricted grant from Research to Prevent Blindness, Inc., by Ophthalrnic Core Grant EY01931, and Vision Research Training Grant T32EY07016, EY05609,
PY - 1990
Y1 - 1990
N2 - A mounting block for in vitro perfusion of the cat cornea is described. Using this apparatus and the techniques of Araie, the permeability (Pac) of the normal cat corneal endothelium to carboxyfluorscein was determined to be 2.5 plusmn; 0.2 × 10-4 cm/min. To assess the sensitivity of this technique in determining changes in Pac associated with alterations in endothelial, morphology, three cats underwent 2 successive unilateral, central, 10 mm diameter circular areas of endothelial debridement 6 weeks apart. Six weeks following the second wounding all 3 animals underwent morphometric analysis and Pac determination. A trend toward an elevation in Pac with extreme reductions in cell density was observed.
AB - A mounting block for in vitro perfusion of the cat cornea is described. Using this apparatus and the techniques of Araie, the permeability (Pac) of the normal cat corneal endothelium to carboxyfluorscein was determined to be 2.5 plusmn; 0.2 × 10-4 cm/min. To assess the sensitivity of this technique in determining changes in Pac associated with alterations in endothelial, morphology, three cats underwent 2 successive unilateral, central, 10 mm diameter circular areas of endothelial debridement 6 weeks apart. Six weeks following the second wounding all 3 animals underwent morphometric analysis and Pac determination. A trend toward an elevation in Pac with extreme reductions in cell density was observed.
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U2 - 10.3109/02713689009003468
DO - 10.3109/02713689009003468
M3 - Article
C2 - 2091891
AN - SCOPUS:0025672789
SN - 0271-3683
VL - 9
SP - 1129
EP - 1136
JO - Current Eye Research
JF - Current Eye Research
IS - 12
ER -