A monoclonal antibody that reacts with nonallelic enzyme glycoproteins

Kathryn J. Gogolin; Larry K. Wray; Clive A. Slaughter; Harry Harris

doi:10.1126/science.6175022

A monoclonal antibody that reacts with nonallelic enzyme glycoproteins

Kathryn J. Gogolin, Larry K. Wray, Clive A. Slaughter, Harry Harris

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

One of six monoclonal antibodies raised against purified human placental alkaline phosphatase cross-reacts with the adult and fetal forms of intestinal alkaline phosphatase. The placental and intestinal enzymes are nonallelic. A new electrophoretic titration procedure was used to assess the relative reactivities of the different enzymes with the antibody. The placental enzyme was the most reactive. However, the adult intestinal enzyme showed greater reactivity than the fetal enzyme. The determinants to which the antibody binds on these three forms of alkaline phosphatase presumably differ in their detailed molecular configurations.

Original language	English (US)
Pages (from-to)	59-61
Number of pages	3
Journal	Science
Volume	216
Issue number	4541
DOIs	https://doi.org/10.1126/science.6175022
State	Published - 1982
Externally published	Yes

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title = "A monoclonal antibody that reacts with nonallelic enzyme glycoproteins",

abstract = "One of six monoclonal antibodies raised against purified human placental alkaline phosphatase cross-reacts with the adult and fetal forms of intestinal alkaline phosphatase. The placental and intestinal enzymes are nonallelic. A new electrophoretic titration procedure was used to assess the relative reactivities of the different enzymes with the antibody. The placental enzyme was the most reactive. However, the adult intestinal enzyme showed greater reactivity than the fetal enzyme. The determinants to which the antibody binds on these three forms of alkaline phosphatase presumably differ in their detailed molecular configurations.",

author = "Gogolin, {Kathryn J.} and Wray, {Larry K.} and Slaughter, {Clive A.} and Harry Harris",

year = "1982",

doi = "10.1126/science.6175022",

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pages = "59--61",

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T1 - A monoclonal antibody that reacts with nonallelic enzyme glycoproteins

AU - Gogolin, Kathryn J.

AU - Wray, Larry K.

AU - Slaughter, Clive A.

AU - Harris, Harry

PY - 1982

Y1 - 1982

N2 - One of six monoclonal antibodies raised against purified human placental alkaline phosphatase cross-reacts with the adult and fetal forms of intestinal alkaline phosphatase. The placental and intestinal enzymes are nonallelic. A new electrophoretic titration procedure was used to assess the relative reactivities of the different enzymes with the antibody. The placental enzyme was the most reactive. However, the adult intestinal enzyme showed greater reactivity than the fetal enzyme. The determinants to which the antibody binds on these three forms of alkaline phosphatase presumably differ in their detailed molecular configurations.

AB - One of six monoclonal antibodies raised against purified human placental alkaline phosphatase cross-reacts with the adult and fetal forms of intestinal alkaline phosphatase. The placental and intestinal enzymes are nonallelic. A new electrophoretic titration procedure was used to assess the relative reactivities of the different enzymes with the antibody. The placental enzyme was the most reactive. However, the adult intestinal enzyme showed greater reactivity than the fetal enzyme. The determinants to which the antibody binds on these three forms of alkaline phosphatase presumably differ in their detailed molecular configurations.

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VL - 216

SP - 59

EP - 61

JO - Science

JF - Science

IS - 4541

ER -

A monoclonal antibody that reacts with nonallelic enzyme glycoproteins

Abstract

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