A nano-sized PARACEST-fluorescence imaging contrast agent facilitates and validates in vivo CEST MRI detection of glioma

Meser M. Ali; Mohammed P.I. Bhuiyan; Branislava Janic; Nadimpalli R.S. Varma; Tom Mikkelsen; James R. Ewing; Robert A. Knight; Mark D. Pagel; Ali S. Arbab

doi:10.2217/nnm.12.92

A nano-sized PARACEST-fluorescence imaging contrast agent facilitates and validates in vivo CEST MRI detection of glioma

Meser M. Ali, Mohammed P.I. Bhuiyan, Branislava Janic, Nadimpalli R.S. Varma, Tom Mikkelsen, James R. Ewing, Robert A. Knight, Mark D. Pagel, Ali S. Arbab

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Aim: The authors have investigated the usefulness of in vivo chemical exchange saturation transfer MRI for detecting gliomas using a dual-modality imaging contrast agent. Materials & methods: A paramagnetic chemical exchange saturation transfer MRI contrast agent, Eu-1,4,7,10- tetraazacclododecane-1,4,7,10-tetraacetic acid-Gly₄ and a fluorescent agent, DyLight^® 680, were conjugated to a generation 5 polyamidoamine dendrimer to create the dual-modality, nano-sized imaging contrast agent. Results: The agent was detected with in vivo chemical exchange saturation transfer MRI in an U87 glioma model. These results were validated using in vivo and ex vivo fluorescence imaging. Conclusion: This study demonstrated the merits of using a nano-sized imaging contrast agent for detecting gliomas and using a dual-modality agent for detecting gliomas at different spatial scales. Original submitted 9 January 2012; Revised submitted 18 April 2012; Published online 14 August 201.

Original language	English (US)
Pages (from-to)	1827-1837
Number of pages	11
Journal	Nanomedicine
Volume	7
Issue number	12
DOIs	https://doi.org/10.2217/nnm.12.92
State	Published - Dec 2012
Externally published	Yes

Keywords

MRI
brain tumor
contrast agent
dendrimer
pharmacokinetic

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Biomedical Engineering
Materials Science(all)

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10.2217/nnm.12.92

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title = "A nano-sized PARACEST-fluorescence imaging contrast agent facilitates and validates in vivo CEST MRI detection of glioma",

abstract = "Aim: The authors have investigated the usefulness of in vivo chemical exchange saturation transfer MRI for detecting gliomas using a dual-modality imaging contrast agent. Materials & methods: A paramagnetic chemical exchange saturation transfer MRI contrast agent, Eu-1,4,7,10- tetraazacclododecane-1,4,7,10-tetraacetic acid-Gly4 and a fluorescent agent, DyLight{\textregistered} 680, were conjugated to a generation 5 polyamidoamine dendrimer to create the dual-modality, nano-sized imaging contrast agent. Results: The agent was detected with in vivo chemical exchange saturation transfer MRI in an U87 glioma model. These results were validated using in vivo and ex vivo fluorescence imaging. Conclusion: This study demonstrated the merits of using a nano-sized imaging contrast agent for detecting gliomas and using a dual-modality agent for detecting gliomas at different spatial scales. Original submitted 9 January 2012; Revised submitted 18 April 2012; Published online 14 August 201.",

keywords = "MRI, brain tumor, contrast agent, dendrimer, pharmacokinetic",

author = "Ali, {Meser M.} and Bhuiyan, {Mohammed P.I.} and Branislava Janic and Varma, {Nadimpalli R.S.} and Tom Mikkelsen and Ewing, {James R.} and Knight, {Robert A.} and Pagel, {Mark D.} and Arbab, {Ali S.}",

year = "2012",

month = dec,

doi = "10.2217/nnm.12.92",

language = "English (US)",

volume = "7",

pages = "1827--1837",

journal = "Nanomedicine",

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AU - Ali, Meser M.

AU - Bhuiyan, Mohammed P.I.

AU - Janic, Branislava

AU - Varma, Nadimpalli R.S.

AU - Mikkelsen, Tom

AU - Ewing, James R.

AU - Knight, Robert A.

AU - Pagel, Mark D.

AU - Arbab, Ali S.

PY - 2012/12

Y1 - 2012/12

N2 - Aim: The authors have investigated the usefulness of in vivo chemical exchange saturation transfer MRI for detecting gliomas using a dual-modality imaging contrast agent. Materials & methods: A paramagnetic chemical exchange saturation transfer MRI contrast agent, Eu-1,4,7,10- tetraazacclododecane-1,4,7,10-tetraacetic acid-Gly4 and a fluorescent agent, DyLight® 680, were conjugated to a generation 5 polyamidoamine dendrimer to create the dual-modality, nano-sized imaging contrast agent. Results: The agent was detected with in vivo chemical exchange saturation transfer MRI in an U87 glioma model. These results were validated using in vivo and ex vivo fluorescence imaging. Conclusion: This study demonstrated the merits of using a nano-sized imaging contrast agent for detecting gliomas and using a dual-modality agent for detecting gliomas at different spatial scales. Original submitted 9 January 2012; Revised submitted 18 April 2012; Published online 14 August 201.

AB - Aim: The authors have investigated the usefulness of in vivo chemical exchange saturation transfer MRI for detecting gliomas using a dual-modality imaging contrast agent. Materials & methods: A paramagnetic chemical exchange saturation transfer MRI contrast agent, Eu-1,4,7,10- tetraazacclododecane-1,4,7,10-tetraacetic acid-Gly4 and a fluorescent agent, DyLight® 680, were conjugated to a generation 5 polyamidoamine dendrimer to create the dual-modality, nano-sized imaging contrast agent. Results: The agent was detected with in vivo chemical exchange saturation transfer MRI in an U87 glioma model. These results were validated using in vivo and ex vivo fluorescence imaging. Conclusion: This study demonstrated the merits of using a nano-sized imaging contrast agent for detecting gliomas and using a dual-modality agent for detecting gliomas at different spatial scales. Original submitted 9 January 2012; Revised submitted 18 April 2012; Published online 14 August 201.

KW - MRI

KW - brain tumor

KW - contrast agent

KW - dendrimer

KW - pharmacokinetic

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A nano-sized PARACEST-fluorescence imaging contrast agent facilitates and validates in vivo CEST MRI detection of glioma

Abstract

Keywords

ASJC Scopus subject areas

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