TY - JOUR
T1 - A novel method of sampling gingival crevicular fluid from a mouse model of periodontitis
AU - Matsuda, Shinji
AU - Movila, Alexandru
AU - Suzuki, Maiko
AU - Kajiya, Mikihito
AU - Wisitrasameewong, Wichaya
AU - Kayal, Rayyan
AU - Hirshfeld, Josefine
AU - Al-Dharrab, Ayman
AU - Savitri, Irma J
AU - Mira, Abdulghani
AU - Kurihara, Hidemi
AU - Taubman, Martin A
AU - Kawai, Toshihisa
N1 - Copyright © 2016. Published by Elsevier B.V.
PY - 2016/11
Y1 - 2016/11
N2 - Using a mouse model of silk ligature-induced periodontal disease (PD), we report a novel method of sampling mouse gingival crevicular fluid (GCF) to evaluate the time-dependent secretion patterns of bone resorption-related cytokines. GCF is a serum transudate containing host-derived biomarkers which can represent cellular response in the periodontium. As such, human clinical evaluations of PD status rely on sampling this critical secretion. At the same time, a method of sampling GCF from mice is absent, hindering the translational value of mouse models of PD. Therefore, we herein report a novel method of sampling GCF from a mouse model of periodontitis, involving a series of easy steps. First, the original ligature used for induction of PD was removed, and a fresh ligature for sampling GCF was placed in the gingival crevice for 10min. Immediately afterwards, the volume of GCF collected in the sampling ligature was measured using a high precision weighing balance. The sampling ligature containing GCF was then immersed in a solution of PBS-Tween 20 and subjected to ELISA. This enabled us to monitor the volume of GCF and detect time-dependent changes in the expression of such cytokines as IL-1b, TNF-α, IL-6, RANKL, and OPG associated with the levels of alveolar bone loss, as reflected in GCF collected from a mouse model of PD. Therefore, this novel GCF sampling method can be used to measure various cytokines in GCF relative to the dynamic changes in periodontal bone loss induced in a mouse model of PD.
AB - Using a mouse model of silk ligature-induced periodontal disease (PD), we report a novel method of sampling mouse gingival crevicular fluid (GCF) to evaluate the time-dependent secretion patterns of bone resorption-related cytokines. GCF is a serum transudate containing host-derived biomarkers which can represent cellular response in the periodontium. As such, human clinical evaluations of PD status rely on sampling this critical secretion. At the same time, a method of sampling GCF from mice is absent, hindering the translational value of mouse models of PD. Therefore, we herein report a novel method of sampling GCF from a mouse model of periodontitis, involving a series of easy steps. First, the original ligature used for induction of PD was removed, and a fresh ligature for sampling GCF was placed in the gingival crevice for 10min. Immediately afterwards, the volume of GCF collected in the sampling ligature was measured using a high precision weighing balance. The sampling ligature containing GCF was then immersed in a solution of PBS-Tween 20 and subjected to ELISA. This enabled us to monitor the volume of GCF and detect time-dependent changes in the expression of such cytokines as IL-1b, TNF-α, IL-6, RANKL, and OPG associated with the levels of alveolar bone loss, as reflected in GCF collected from a mouse model of PD. Therefore, this novel GCF sampling method can be used to measure various cytokines in GCF relative to the dynamic changes in periodontal bone loss induced in a mouse model of PD.
KW - Alveolar Bone Loss/etiology
KW - Animals
KW - Biomarkers/analysis
KW - Cytokines/analysis
KW - Disease Models, Animal
KW - Gingival Crevicular Fluid/chemistry
KW - Humans
KW - Ligation
KW - Maxilla/pathology
KW - Mice
KW - Mice, Inbred C57BL
KW - Periodontitis/pathology
KW - Specimen Handling/methods
U2 - 10.1016/j.jim.2016.08.008
DO - 10.1016/j.jim.2016.08.008
M3 - Article
C2 - 27589925
SN - 0022-1759
VL - 438
SP - 21
EP - 25
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
ER -
