A novel method of sampling gingival crevicular fluid from a mouse model of periodontitis

Shinji Matsuda, Alexandru Movila, Maiko Suzuki, Mikihito Kajiya, Wichaya Wisitrasameewong, Rayyan Kayal, Josefine Hirshfeld, Ayman Al-Dharrab, Irma J Savitri, Abdulghani Mira, Hidemi Kurihara, Martin A Taubman, Toshihisa Kawai

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Using a mouse model of silk ligature-induced periodontal disease (PD), we report a novel method of sampling mouse gingival crevicular fluid (GCF) to evaluate the time-dependent secretion patterns of bone resorption-related cytokines. GCF is a serum transudate containing host-derived biomarkers which can represent cellular response in the periodontium. As such, human clinical evaluations of PD status rely on sampling this critical secretion. At the same time, a method of sampling GCF from mice is absent, hindering the translational value of mouse models of PD. Therefore, we herein report a novel method of sampling GCF from a mouse model of periodontitis, involving a series of easy steps. First, the original ligature used for induction of PD was removed, and a fresh ligature for sampling GCF was placed in the gingival crevice for 10min. Immediately afterwards, the volume of GCF collected in the sampling ligature was measured using a high precision weighing balance. The sampling ligature containing GCF was then immersed in a solution of PBS-Tween 20 and subjected to ELISA. This enabled us to monitor the volume of GCF and detect time-dependent changes in the expression of such cytokines as IL-1b, TNF-α, IL-6, RANKL, and OPG associated with the levels of alveolar bone loss, as reflected in GCF collected from a mouse model of PD. Therefore, this novel GCF sampling method can be used to measure various cytokines in GCF relative to the dynamic changes in periodontal bone loss induced in a mouse model of PD.

Original languageEnglish (US)
Pages (from-to)21-25
Number of pages5
JournalJournal of Immunological Methods
StatePublished - Nov 2016
Externally publishedYes


  • Alveolar Bone Loss/etiology
  • Animals
  • Biomarkers/analysis
  • Cytokines/analysis
  • Disease Models, Animal
  • Gingival Crevicular Fluid/chemistry
  • Humans
  • Ligation
  • Maxilla/pathology
  • Mice
  • Mice, Inbred C57BL
  • Periodontitis/pathology
  • Specimen Handling/methods


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