A Uve1p-mediated mismatch repair pathway in Schizosaccharomyces pombe

Balveen Kaur, J. Lee A. Fraser, Greg A. Freyer, Scott Davey, Paul W. Doetsch

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


UV damage endonuclease (Uve1p) from Schizosaccharomyces pombe was initially described as a DNA repair enzyme specific for the repair of UV light-induced photoproducts and proposed as the initial step in an alternative excision repair pathway. Here we present biochemical and genetic evidence demonstrating that Uve1p is also a mismatch repair endonuclease which recognizes and cleaves DNA 5' to the mispaired base in a strand- specific manner. The biochemical properties of the Uve1p-mediated mismatch endonuclease activity are similar to those of the Uve1p-mediated UV photoproduct endonuclease. Mutants lacking Uve1p display a spontaneous mutator phenotype, further confirming the notion that Uve1p plays a role in mismatch repair. These results suggest that Uve1p has a surprisingly broad substrate specificity and may function as a general type of DNA repair protein with the capacity to initiate mismatch repair in certain organisms.

Original languageEnglish (US)
Pages (from-to)4703-4710
Number of pages8
JournalMolecular and Cellular Biology
Issue number7
StatePublished - Jul 1999
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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