TY - JOUR
T1 - Accumulation of A Globo-series Glycolipid Having Galα 1–3 Gal In Pc12h Pheochromocytoma Cells
AU - Ariga, Toshio
AU - Yu, Robert K.
AU - Scarsdale, J. Neel
AU - Suzuki, Minoru
AU - Kuroda, Yoichiro
AU - Kitagawa, Hisayo
AU - Miyatake, Tadashi
PY - 1988/7/1
Y1 - 1988/7/1
N2 - In a previous paper, we reported the presence of globoside as a major neutral glycolipid in PC 12 pheochromocytoma cells [Ariga, T., Macala, L. J., Saito, M., Margolis, R. K., Greene, L. A., Margolis, R. U., & Yu, R. K. (1988) Biochemistry 27, 52–58], Recently, we found that subcloned PC12h cells accumulated another unusual neutral glycolipid. In order to characterize this glycolipid, PC12h cells were subcutaneously transplanted into rats. The induced tumor tissue accumulated two major neutral glycolipids, which were purified by Iatrobeads column and preparative thin-layer chromatographies. One of the glycolipids was found to be globoside, and the other had a globotriaosyl structure with an additional terminal Galα 1–3 residue. Its structure was determined by fast atom bombardment mass spectrometry, two-dimensional proton nuclear magnetic resonance spectrometry (2D NMR), permethylation study, sequential degradation with exoglycosidase, and mild acid hydrolysis to be Gal(αl-3)Gal(αl-4)Gal(β1-4)Glc(β1-1′)Cer.
AB - In a previous paper, we reported the presence of globoside as a major neutral glycolipid in PC 12 pheochromocytoma cells [Ariga, T., Macala, L. J., Saito, M., Margolis, R. K., Greene, L. A., Margolis, R. U., & Yu, R. K. (1988) Biochemistry 27, 52–58], Recently, we found that subcloned PC12h cells accumulated another unusual neutral glycolipid. In order to characterize this glycolipid, PC12h cells were subcutaneously transplanted into rats. The induced tumor tissue accumulated two major neutral glycolipids, which were purified by Iatrobeads column and preparative thin-layer chromatographies. One of the glycolipids was found to be globoside, and the other had a globotriaosyl structure with an additional terminal Galα 1–3 residue. Its structure was determined by fast atom bombardment mass spectrometry, two-dimensional proton nuclear magnetic resonance spectrometry (2D NMR), permethylation study, sequential degradation with exoglycosidase, and mild acid hydrolysis to be Gal(αl-3)Gal(αl-4)Gal(β1-4)Glc(β1-1′)Cer.
UR - http://www.scopus.com/inward/record.url?scp=0023726401&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023726401&partnerID=8YFLogxK
U2 - 10.1021/bi00414a058
DO - 10.1021/bi00414a058
M3 - Article
C2 - 3167050
AN - SCOPUS:0023726401
SN - 0006-2960
VL - 27
SP - 5335
EP - 5340
JO - Biochemistry
JF - Biochemistry
IS - 14
ER -