Addition of phosphates and chlorhexidine to resin-modified MTA materials

Rafael Pino Vitti, Rafael Rocha Pacheco, Emmanuel João Nogueira Leal Silva, Carlo Prati, Maria Giovanna Gandolfi, Evandro Piva, Fabrício Aulo Ogliari, Cesar Henrique Zanchi, Mário Alexandre Coelho Sinhoreti

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


To evaluate the properties of experimental mineral trioxide aggregate (MTA) resin-modified materials for root-end filling procedures, varying their compositions regarding the addition of hydroxiapatite (HA) or dicalcium phosphate dihydrate, with or without chlorhexidine digluconate. White MTA (Angelus, Londrina, Brazil) was used as a reference material. Degree of conversion (DC) was evaluated by Fourier transformed infrared (FTIr) spectroscopy (n = 5). Flowability (n = 3) and radiopacity (n = 3) were evaluated following ISO 6876:2001 methods. For splitting tensile strength analysis, cylindrical samples (n = 10) were subjected to compressive load using a universal testing machine (Instron Corporation, Norwood, MA). Water sorption and solubility tests were performed according to ISO 4049:2009 methods. Calcium ion release and pH analysis (n = 10) were evaluated using a pH meter (Orion, Watsonville, CA). Cytotoxicity (n = 8) of materials extracts was evaluated as cell viability percentage. Statistical analysis was performed using Kolmogorov–Smirnov for normal distribution and data was subjected to one-way ANOVA and Tukey test (α = 0.05). Addition of chlorhexidine digluconate reduced DC mean values for experimental materials (<50%). White MTA demonstrated lower flowability (5.3 mm) and higher radiopacity (9.8 mm Al), splitting tensile strength (9.1 MPa), solubility (8.2 μg/mm3), calcium ion release (~26.5 ppm), cytotoxicity (55.2%), and pH mean values (10.8), when compared to experimental materials. All groups demonstrated a decrease in calcium release (<85%) and pH (<13%). Formulation containing HA demonstrated similar pH values after 28 days when compared to white MTA. Evaluated experimental resin-modified MTA based materials without chlorhexidine digluconate showed satisfactory results for all physico-chemical properties tested and cytotoxicity.

Original languageEnglish (US)
Pages (from-to)2195-2201
Number of pages7
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Issue number6
StatePublished - Aug 2019
Externally publishedYes


  • endodontics
  • hydroxyapatite
  • methacrylates
  • mineralized tissue/development
  • regeneration

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering


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