TY - JOUR
T1 - Antimicrobial efficacy of 3.8% silver diamine fluoride and its effect on root dentin
AU - Hiraishi, Noriko
AU - Yiu, Cynthia K.Y.
AU - King, Nigel M.
AU - Tagami, Junji
AU - Tay, Franklin Chi Meng
PY - 2010/4/23
Y1 - 2010/4/23
N2 -
Introduction: This study investigated the use of 3.8% silver diamine fluoride (Ag[NH
3
]
2
F) as an antibacterial agent against Enterococcus faecalis biofilms and its ability to penetrate dentinal tubules by the formation of silver salts. Methods: Biofims were generated on membrane filter discs and subjected to 15-minute and 60-minute exposure times with 3.8% Ag(NH
3
)
2
F, saturated Ca(OH)
2
, 5.25% NaOCl (negative control), and 0.9% NaCl (positive control). Cleaned and shaped radicular dentin were applied with Ag(NH
3
)
2
F for 24, 48, and 72 hours. The presence of silver salts on the dentin surface was examined using low-pressure scanning electron microscopy. Results: Both NaOCl and Ag(NH
3
)
2
F were effective against E. faecalis biofilms, with no significant difference in reduction of microorganisms for both exposure times. Silver deposits were present on 66.5% of the radicular dentin surfaces after 72-hour application of Ag(NH
3
)
2
F as simulated interappointment dressings. Penetration of the silver deposits was observed at most 40 μm into dentinal tubules after smear layer removal. Conclusion: Ag(NH
3
)
2
F has potential to be used as an antimicrobial root canal irrigant or interappointment dressing, especially in locations in which potential browning/blackening of dentin by metallic silver is not a major concern.
AB -
Introduction: This study investigated the use of 3.8% silver diamine fluoride (Ag[NH
3
]
2
F) as an antibacterial agent against Enterococcus faecalis biofilms and its ability to penetrate dentinal tubules by the formation of silver salts. Methods: Biofims were generated on membrane filter discs and subjected to 15-minute and 60-minute exposure times with 3.8% Ag(NH
3
)
2
F, saturated Ca(OH)
2
, 5.25% NaOCl (negative control), and 0.9% NaCl (positive control). Cleaned and shaped radicular dentin were applied with Ag(NH
3
)
2
F for 24, 48, and 72 hours. The presence of silver salts on the dentin surface was examined using low-pressure scanning electron microscopy. Results: Both NaOCl and Ag(NH
3
)
2
F were effective against E. faecalis biofilms, with no significant difference in reduction of microorganisms for both exposure times. Silver deposits were present on 66.5% of the radicular dentin surfaces after 72-hour application of Ag(NH
3
)
2
F as simulated interappointment dressings. Penetration of the silver deposits was observed at most 40 μm into dentinal tubules after smear layer removal. Conclusion: Ag(NH
3
)
2
F has potential to be used as an antimicrobial root canal irrigant or interappointment dressing, especially in locations in which potential browning/blackening of dentin by metallic silver is not a major concern.
KW - Antimicrobial
KW - Enterococcus faecalis
KW - irrigant
KW - root canal medicament
KW - silver diamine fluoride
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U2 - 10.1016/j.joen.2010.02.029
DO - 10.1016/j.joen.2010.02.029
M3 - Article
C2 - 20478459
AN - SCOPUS:77955306808
SN - 0099-2399
VL - 36
SP - 1026
EP - 1029
JO - Journal of Endodontics
JF - Journal of Endodontics
IS - 6
ER -