Application of biologically-oriented dentin bonding principles to the use of endodontic irrigants

Franklin García-Godoy, Robert J. Loushine, Anut Itthagarun, R. Norman Weller, Peter E. Murray, Albert J. Feilzer, David H. Pashley, Franklin R. Tay

Research output: Contribution to journalArticlepeer-review

51 Scopus citations


Purpose: To compare the removal of smear layer and the structure of the hybrid layer formed after the use of EDTA or MTAD solutions when used as a final flush. Methods: Single-rooted extracted premolars (n=18) were collected and treated for root canal therapy using NaOCl irrigation followed by a final rinse of 17% EDTA, Biopure MTAD, or saline (negative control). The roots were obturated with gutta-percha and a hydrophilic HEMA-containing root canal sealer. The TEM specimens were impregnated with 50% silver nitrate to visualize sealing imperfections and nanoleakage. The structure of the coronal, middle and apical parts of root canal walls was examined using transmission electron microscopy. Results: After NaOCl irrigation, a final rinse with BioPure MTAD or 17% EDTA completely removed the 2 μm-thick smear layer on mechanically instrumented root canal walls. The BioPure MTAD hybrid layer was thicker than the 17% EDTA hybrid layer. Both the BioPure MTAD and EDTA caused a collapse of the dentin matrix structure which impeded sealer infiltration and the formation of high quality hybrid layer bonding. The hybrid layers created in smear layer-covered dentin exhibited less potential for nanoleakage than the MTAD or EDTA hybrid layers.

Original languageEnglish (US)
Pages (from-to)281-290
Number of pages10
JournalAmerican journal of dentistry
Issue number4
StatePublished - Aug 2005

ASJC Scopus subject areas

  • Dentistry(all)


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