@article{db9fb36332d041e299eaec3a5af931a0,
title = "Arp2/3 and Mena/VASP Require Profilin 1 for Actin Network Assembly at the Leading Edge",
abstract = "How do different actin structures assemble from a common monomer pool? Skruber et al. show that the monomer-binding protein PFN1 is required for Arp2/3 and Mena/VASP network assembly at the leading edge. Altering PFN1 concentration changes how these networks compete and collaborate, driving the formation of different leading-edge actin structures.",
keywords = "Arp2/3, Mena/VASP, actin, assembly, filopodia, lamellipodia, leading edge, monomer, polymerization, profilin",
author = "Kristen Skruber and Warp, {Peyton V.} and Rachael Shklyarov and Thomas, {James D.} and Swanson, {Maurice S.} and Henty-Ridilla, {Jessica L.} and Read, {Tracy Ann} and Vitriol, {Eric A.}",
note = "Funding Information: We would like to thank Dr. Alpha Yap and Suzie Verma for the generous gift of the EGFP-FP4-Mito and EGFP-AP4-Mito DNA constructs. This project was supported by a National Institutes of Health (NIH) Maximizing Investigators{\textquoteright} Research Award for Early Stage Investigators (R35GM133485) to J.L.H.-R. and an NIH Pathway to Independence Award (R00 NS087104) to E.A.V. We would also like to thank Dr. Stephen P. Sugrue and The UF College of Medicine Office of Research Affairs for their support. The authors wish to thank the anonymous reviewers for their insight and suggestions. Conceptualization, K.S. and E.A.V.; Methodology, K.S. and E.A.V.; Formal Analysis, K.S. P.V.W. R.S. J.D.T. and E.V.; Investigation, K.S. and E.A.V.; Resources, K.S. M.S.S. and J.L.H.-R.; Writing – Original Draft, K.S. and E.A.V.; Writing – Review and Editing, K.S. T.-A.R. and E.A.V.; Visualization, K.S. P.V.W. and E.A.V.; Project Administration, E.A.V.; Supervision, T.-A.R. and E.A.V.; Funding Acquisition, E.A.V. The authors declare no competing interests. Funding Information: We would like to thank Dr. Alpha Yap and Suzie Verma for the generous gift of the EGFP-FP4-Mito and EGFP-AP4-Mito DNA constructs. This project was supported by a National Institutes of Health ( NIH ) Maximizing Investigators{\textquoteright} Research Award for Early Stage Investigators ( R35GM133485 ) to J.L.H.-R. and an NIH Pathway to Independence Award ( R00 NS087104 ) to E.A.V. We would also like to thank Dr. Stephen P. Sugrue and The UF College of Medicine Office of Research Affairs for their support. The authors wish to thank the anonymous reviewers for their insight and suggestions. Publisher Copyright: {\textcopyright} 2020 The Author(s)",
year = "2020",
month = jul,
day = "20",
doi = "10.1016/j.cub.2020.04.085",
language = "English (US)",
volume = "30",
pages = "2651--2664.e5",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "14",
}