TY - JOUR
T1 - Cadmium reduces nitric oxide production by impairing phosphorylation of endothelial nitric oxide synthase
AU - Majumder, Syamantak
AU - Muley, Ajit
AU - Kolluru, Gopi Krishna
AU - Saurabh, Samir
AU - Tamilarasan, K. P.
AU - Chandrasekhar, Sidhharth
AU - Reddy, Hima Bindu
AU - Purohit, Sharad
AU - Chatterjee, Suvro
PY - 2008/2
Y1 - 2008/2
N2 - Cadmium (Cd) perturbs vascular health and interferes with endothelial function. However, the effects of exposing endothelial cells to low doses of Cd on the production of nitric oxide (NO) are largely unknown. The objective of the present study was to evaluate these effects by using low levels of CdCl 2 concentrations, ranging from 10 to 1000 nmol/L. Cd perturbations in endothelial function were studied by employing wound-healing and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. The results suggest that a CdCl2 concentration of 100 nmol/L maximally attenuated NO production, cellular migration, and energy metabolism in endothelial cells. An egg yolk angiogenesis model was employed to study the effect of Cd exposure on angiogenesis. The results demonstrate that NO supplementation restored Cd-attenuated angiogenesis. Immunofluorescence, Western blot, and immuno-detection studies showed that low levels of Cd inhibit NO production in endothelial cells by blocking eNOS phosphorylation, which is possibly linked to processes involving endothelial function and dysfunction, including angiogenesis.
AB - Cadmium (Cd) perturbs vascular health and interferes with endothelial function. However, the effects of exposing endothelial cells to low doses of Cd on the production of nitric oxide (NO) are largely unknown. The objective of the present study was to evaluate these effects by using low levels of CdCl 2 concentrations, ranging from 10 to 1000 nmol/L. Cd perturbations in endothelial function were studied by employing wound-healing and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. The results suggest that a CdCl2 concentration of 100 nmol/L maximally attenuated NO production, cellular migration, and energy metabolism in endothelial cells. An egg yolk angiogenesis model was employed to study the effect of Cd exposure on angiogenesis. The results demonstrate that NO supplementation restored Cd-attenuated angiogenesis. Immunofluorescence, Western blot, and immuno-detection studies showed that low levels of Cd inhibit NO production in endothelial cells by blocking eNOS phosphorylation, which is possibly linked to processes involving endothelial function and dysfunction, including angiogenesis.
KW - Angiogenesis
KW - Cadmium
KW - Endothelial cells
KW - Nitric oxide
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U2 - 10.1139/O07-146
DO - 10.1139/O07-146
M3 - Article
C2 - 18364740
AN - SCOPUS:39049156703
SN - 0829-8211
VL - 86
SP - 1
EP - 10
JO - Biochemistry and Cell Biology
JF - Biochemistry and Cell Biology
IS - 1
ER -