TY - JOUR
T1 - Clerodendron glandulosum.Coleb extract prevents in vitro human LDL oxidation and oxidized LDL induced apoptosis in human monocyte derived macrophages
AU - Jadeja, Ravirajsinh N.
AU - Thounaojam, Menaka C.
AU - Devkar, Ranjisinh V.
AU - Ramachandran, A. V.
PY - 2011/6
Y1 - 2011/6
N2 - This study reports the protective role of Clerodendron glandulosum (CG) extract against in vitro LDL oxidation and Ox-LDL induced macrophage apoptosis using various experimental models. Effect of CG extract on Cu2+ mediated LDL oxidation kinetics and formation of various intermediary products and its ability to prevent human monocyte derived macrophage mediated LDL oxidation have been investigated. Ox-LDL induced macrophage apoptosis was evaluated by nuclear condensation, cell cycle analysis, and annexin V-FITC/PI staining in presence or absence of CG extract. Results recorded in the present study clearly suggest the protective role of CG extract against LDL oxidation and Ox-LDL induced macrophage oxidative stress, mitochondrial dysfunction, and apoptosis. This is the first report on the protective role of CG extract on two key events of atherosclerosis portending its possible therapeutic use as an anti-atherogenic herbal medicine.
AB - This study reports the protective role of Clerodendron glandulosum (CG) extract against in vitro LDL oxidation and Ox-LDL induced macrophage apoptosis using various experimental models. Effect of CG extract on Cu2+ mediated LDL oxidation kinetics and formation of various intermediary products and its ability to prevent human monocyte derived macrophage mediated LDL oxidation have been investigated. Ox-LDL induced macrophage apoptosis was evaluated by nuclear condensation, cell cycle analysis, and annexin V-FITC/PI staining in presence or absence of CG extract. Results recorded in the present study clearly suggest the protective role of CG extract against LDL oxidation and Ox-LDL induced macrophage oxidative stress, mitochondrial dysfunction, and apoptosis. This is the first report on the protective role of CG extract on two key events of atherosclerosis portending its possible therapeutic use as an anti-atherogenic herbal medicine.
KW - Apoptosis
KW - Clerodenron glandulosum.Coleb
KW - LDL oxidation
KW - Macrophage
KW - Oxidative stress
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UR - http://www.scopus.com/inward/citedby.url?scp=79955665037&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2011.02.017
DO - 10.1016/j.fct.2011.02.017
M3 - Article
C2 - 21371519
AN - SCOPUS:79955665037
SN - 0278-6915
VL - 49
SP - 1195
EP - 1202
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
IS - 6
ER -
