Contribution of biomimetic collagen-ligand interaction to intrafibrillar mineralization

Q. Song, K. Jiao, L. Tonggu, L. G. Wang, S. L. Zhang, Y. D. Yang, L. Zhang, J. H. Bian, D. X. Hao, C. Y. Wang, Y. X. Ma, D. D. Arola, L. Breschi, J. H. Chen, Franklin Chi Meng Tay, L. N. Niu

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55 Scopus citations


Contemporary models of intrafibrillar mineralization mechanisms are established using collagen fibrils as templates without considering the contribution from collagen-bound apatite nucleation inhibitors. However, collagen matrices destined for mineralization in vertebrates contain bound matrix proteins for intrafibrillar mineralization. Negatively charged, high–molecular weight polycarboxylic acid is cross-linked to reconstituted collagen to create a model for examining the contribution of collagen-ligand interaction to intrafibrillar mineralization. Cryogenic electron microscopy and molecular dynamics simulation show that, after cross-linking to collagen, the bound polyelectrolyte caches prenucleation cluster singlets into chain-like aggregates along the fibrillar surface to increase the pool of mineralization precursors available for intrafibrillar mineralization. Higher-quality mineralized scaffolds with better biomechanical properties are achieved compared with mineralization of unmodified scaffolds in polyelectrolyte-stabilized mineralization solution. Collagen-ligand interaction provides insights on the genesis of heterogeneously mineralized tissues and the potential causes of ectopic calcification in nonmineralized body tissues.

Original languageEnglish (US)
Article numbereaav9075
JournalScience Advances
Issue number3
StatePublished - Mar 29 2019

ASJC Scopus subject areas

  • General


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