TY - JOUR
T1 - Cutting edge
T2 - Induced indoleamine 2,3 dioxygenase expression in dendritic cell subsets suppresses T cell clonal expansion
AU - Mellor, Andrew L.
AU - Baban, Babak
AU - Chandler, Phillip
AU - Marshall, Brendan
AU - Jhaver, Kanchan
AU - Hansen, Anna
AU - Koni, Pandelakis A.
AU - Iwashima, Makio
AU - Munn, David H.
PY - 2003/8/15
Y1 - 2003/8/15
N2 - In mice, immunoregulatory APCs express the dendritic cell (DC) marker CD11c, and one or more distinctive markers (CD8α, B220, DX5). In this study, we show that expression of the tryptophan-degrading enzyme indoleamine 2,3 dioxygenase (IDO) is selectively induced in specific splenic DC subsets when mice were exposed to the synthetic immunomodulatory reagent CTLA4-Ig. CTLA4-Ig did not induce IDO expression in macrophages or lymphoid cells. Induction of IDO completely blocked clonal expansion of T cells from TCR transgenic mice following adoptive transfer, whereas CTLA4-Ig treatment did not block T cell clonal expansion in IDO-deficient recipients. Thus, IDO expression is an inducible feature of specific subsets of DCs, and provides a potential mechanistic explanation for their T cell regulatory properties.
AB - In mice, immunoregulatory APCs express the dendritic cell (DC) marker CD11c, and one or more distinctive markers (CD8α, B220, DX5). In this study, we show that expression of the tryptophan-degrading enzyme indoleamine 2,3 dioxygenase (IDO) is selectively induced in specific splenic DC subsets when mice were exposed to the synthetic immunomodulatory reagent CTLA4-Ig. CTLA4-Ig did not induce IDO expression in macrophages or lymphoid cells. Induction of IDO completely blocked clonal expansion of T cells from TCR transgenic mice following adoptive transfer, whereas CTLA4-Ig treatment did not block T cell clonal expansion in IDO-deficient recipients. Thus, IDO expression is an inducible feature of specific subsets of DCs, and provides a potential mechanistic explanation for their T cell regulatory properties.
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U2 - 10.4049/jimmunol.171.4.1652
DO - 10.4049/jimmunol.171.4.1652
M3 - Article
C2 - 12902462
AN - SCOPUS:0042591467
SN - 0022-1767
VL - 171
SP - 1652
EP - 1655
JO - Journal of Immunology
JF - Journal of Immunology
IS - 4
ER -