Abstract
The enzymatic activity and distribution of peroxisomes (microbodies) in rat and guinea pig hearts were studied cytochemically, by means of oxidation of 3-3′-diaminobenzidine (DAB) and by using B-glycerophosphate and cytidine-5′-monophosphate as substrates. Peroxisomes were localized in proximity to mitochondria and sarcoplasmic reticulum and measured from 0.2 μrn to 0.5 μm in diameter in both animal species. DAB positive bodies were seen both at pH 9.0 and pH 5.0 in rat myocardial cells. However, in guinea pig myocardial cells the reaction was observed only at pH 9.0, or very faintly at pH 5.0. Acid and alkaline phosphatases were not demonstrated in the peroxisomes. Lipid droplets were surrounded by a ring of dense granular reaction product for enzymes, such as acid and alkaline phosphatase, and lipofuscin granules were limited by acid phosphatase or DAB reaction products. The pathophysiological function of peroxisomes is discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 205-212 |
| Number of pages | 8 |
| Journal | Virchows Archiv B Cell Pathology Including Molecular Pathology |
| Volume | 34 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 1980 |
| Externally published | Yes |
Keywords
- Acid phosphatase
- Alkaline phosphatase
- Catalase
- Lipid metabolism
- Microbodies
- Myocardium
- Peroxisome
- Sarcoplasmic reticulum
ASJC Scopus subject areas
- Pathology and Forensic Medicine