Deleterious effects of fungizone on growth hormone and prolactin secretion by cultured GH3 cells

C. A. Lapp, J. M. Tyler, M. E. Stachura, Y. S. Lee

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


The rates at which growth hormone (GH) and prolactin (PRL) are spontaneously secreted from a rat pituitary tumor cell line (GH3) were significantly reduced when these cells were maintained in medium containing 2.5 μg/ml Fungizone (Fz). The reduction in hormone secretion was not immediately reversed by removal of Fz during perifusion, but after 3 wk in control medium, secretory rates approached the pre-Fz treatment levels. In plated cells, secretion of GH was reduced by Fz in a dose-dependent manner, whereas PRL secretion was significantly reduced only by the highest concentration (2.5 μg/ml) of Fz. We concluded that Fz is not an acceptable medium constituent for the long-term culture of GH3 cells. However, because its effects are reversible, its short-term use as a decontaminating agent might eliminate the necessity for reinitiating the culture of cells whose secretory behavior must be followed in long-term protocols.

Original languageEnglish (US)
Pages (from-to)837-840
Number of pages4
JournalIn Vitro Cellular & Developmental Biology
Issue number12
StatePublished - Dec 1 1987


  • GH
  • fungizone
  • growth hormone
  • prolactin

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology


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