TY - JOUR
T1 - Development and validation of a method for creating incomplete vertical root fracture in extracted teeth
AU - de Lima Dias-Junior, Luiz Carlos
AU - Corrêa, Marcio
AU - da Silveira Teixeira, Cleonice
AU - de Souza, Diego Leonardo
AU - Tay, Franklin R.
AU - Estrela, Carlos
AU - da Fonseca Roberti Garcia, Lucas
AU - Bortoluzzi, Eduardo Antunes
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive licence to The Society of The Nippon Dental University.
PY - 2023/7
Y1 - 2023/7
N2 - The present study reported a method for inducing incomplete root fracture in human extracted teeth for the purpose of evaluating the merits of different diagnostic imaging techniques. Thirty-five single-rooted teeth were inspected under magnification and transillumination to exclude previously fractured teeth. Tooth crowns were removed, and the root canals were prepared up to the ProTaper Next X4 (40.06) file. Each root was lined with wax and embedded in a polystyrene resin block. The setup was attached to a universal testing machine for pressing a customized conical wedge (diameter at tip: 0.6 mm; taper: 0.2 mm/mm) into the instrumented canal with a 2 kN load at 5 mm/min. The machine was programmed to stop after a sudden 10% drop in loading force. Each specimen was removed from the resin block and inspected under × 20 magnification and transillumination to identify the fracture characteristics (pattern, surfaces and root-third affected). The gap width of each specimen was measured at different locations along the fracture line. The protocol induced incomplete vertical root fractures in all specimens. Fracture widths were < 100 μm in all specimens (mean gap width: 34.9 μm). The proposed methodology was successful in inducing incomplete vertical root fractures with characteristics that resemble the clinical presentation of these conditions. The method is easy to execute, highly reproducible and helps to minimize bias in laboratory studies that aims to mimic vertical root fractures.
AB - The present study reported a method for inducing incomplete root fracture in human extracted teeth for the purpose of evaluating the merits of different diagnostic imaging techniques. Thirty-five single-rooted teeth were inspected under magnification and transillumination to exclude previously fractured teeth. Tooth crowns were removed, and the root canals were prepared up to the ProTaper Next X4 (40.06) file. Each root was lined with wax and embedded in a polystyrene resin block. The setup was attached to a universal testing machine for pressing a customized conical wedge (diameter at tip: 0.6 mm; taper: 0.2 mm/mm) into the instrumented canal with a 2 kN load at 5 mm/min. The machine was programmed to stop after a sudden 10% drop in loading force. Each specimen was removed from the resin block and inspected under × 20 magnification and transillumination to identify the fracture characteristics (pattern, surfaces and root-third affected). The gap width of each specimen was measured at different locations along the fracture line. The protocol induced incomplete vertical root fractures in all specimens. Fracture widths were < 100 μm in all specimens (mean gap width: 34.9 μm). The proposed methodology was successful in inducing incomplete vertical root fractures with characteristics that resemble the clinical presentation of these conditions. The method is easy to execute, highly reproducible and helps to minimize bias in laboratory studies that aims to mimic vertical root fractures.
KW - Cone-beam computed tomography
KW - Diagnosis
KW - Endodontics
KW - Fracture width
KW - Vertical root fracture
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U2 - 10.1007/s10266-023-00787-z
DO - 10.1007/s10266-023-00787-z
M3 - Article
C2 - 36708433
AN - SCOPUS:85146938651
SN - 1618-1247
VL - 111
SP - 750
EP - 758
JO - Odontology
JF - Odontology
IS - 3
ER -