TY - JOUR
T1 - DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis
AU - Liu, Xuesong
AU - Zou, Hua
AU - Slaughter, Clive
AU - Wang, Xiaodong
N1 - Funding Information:
Correspondence regarding this paper should be addressed to X. W. We thank Caryn Naekyung Kim and Alexis Lutschg for excellent technical assistance. We thank Carolyn Moomaw and Steve Afendis for help with the protein sequencing analysis and Dr. Bikash Peamanik for assistance with the mass spectrometry analysis. We are grateful to our colleagues Drs. Joseph Goldstein and Michael Brown for critically reading the manuscript. Suspension-cultured HeLa cells were obtained from the Cell Culture Center in Minneapolis, Minnesota. X. W. is supported by an American Cancer Society Research Grant and a Junior Faculty Award.
PY - 1997/4/18
Y1 - 1997/4/18
N2 - We have identified and purified from HeLa cytosol a protein that induces DNA fragmentation in coincubated nuclei after it is activated by caspase-3. This protein, designated DNA Fragmentation Factor (DFF), is a heterodimer of 40 kDa and 45 kDa subunits. The amino acid sequence of the 45 kDa subunit, determined from its cDNA sequence, reveals it to be a novel protein. Caspase- 3 cleaves the 45 kDa subunit at two sites to generate an active factor that produces DNA fragmentation without further requirement for caspase-3 or other cytosolic proteins. In cells undergoing apoptosis, the 45 kDa subunit is cleaved in the same pattern as it is cleaved by caspase-3 in vitro. These data delineate a direct signal transduction pathway during apoptosis: caspase-3 to DFF to DNA fragmentation.
AB - We have identified and purified from HeLa cytosol a protein that induces DNA fragmentation in coincubated nuclei after it is activated by caspase-3. This protein, designated DNA Fragmentation Factor (DFF), is a heterodimer of 40 kDa and 45 kDa subunits. The amino acid sequence of the 45 kDa subunit, determined from its cDNA sequence, reveals it to be a novel protein. Caspase- 3 cleaves the 45 kDa subunit at two sites to generate an active factor that produces DNA fragmentation without further requirement for caspase-3 or other cytosolic proteins. In cells undergoing apoptosis, the 45 kDa subunit is cleaved in the same pattern as it is cleaved by caspase-3 in vitro. These data delineate a direct signal transduction pathway during apoptosis: caspase-3 to DFF to DNA fragmentation.
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U2 - 10.1016/S0092-8674(00)80197-X
DO - 10.1016/S0092-8674(00)80197-X
M3 - Article
C2 - 9108473
AN - SCOPUS:0030916417
SN - 0092-8674
VL - 89
SP - 175
EP - 184
JO - Cell
JF - Cell
IS - 2
ER -