TY - JOUR
T1 - Distinct effects of different phosphatidylglycerol species on mouse keratinocyte proliferation
AU - Xie, Ding
AU - Seremwe, Mutsa
AU - Edwards, John G.
AU - Podolsky, Robert
AU - Bollag, Wendy B.
N1 - Publisher Copyright:
© 2014 PLOS ONE.
PY - 2014/9/18
Y1 - 2014/9/18
N2 - We have previously shown that liposomes composed of egg-derived phosphatidylglycerol (PG), with a mixed fatty acid composition (comprising mainly palmitate and oleate), inhibit the proliferation and promote the differentiation of rapidly dividing keratinocytes, and stimulate the growth of slowly proliferating epidermal cells. To determine the species of PG most effective at modulating keratinocyte proliferation, primary mouse keratinocytes were treated with different PG species, and proliferation was measured. PG species containing polyunsaturated fatty acids were effective at inhibiting rapidly proliferating keratinocytes, whereas PG species with monounsaturated fatty acids were effective at promoting proliferation in slowly dividing cells. Thus, palmitoyl-arachidonyl-PG (16:0/20:4), palmitoyl-linoleoyl-PG (16:0/18:2), dilinoleoyl-PG (18:2/18:2) and soy PG (a PG mixture with a large percentage of polyunsaturated fatty acids) were particularly effective at inhibiting proliferation in rapidly dividing keratinocytes. Conversely, palmitoyl-oleoyl-PG (16:0/18:1) and dioleoyl-PG (18:1/18:1) were especially effective proproliferative PG species. This result represents the first demonstration of opposite effects of different species of a single class of phospholipid and suggests that these different PG species may signal to diverse effector enzymes to differentially affect keratinocyte proliferation and normalize keratinocyte proliferation. Thus, different PG species may be useful for treating skin diseases characterized by excessive or insufficient proliferation.
AB - We have previously shown that liposomes composed of egg-derived phosphatidylglycerol (PG), with a mixed fatty acid composition (comprising mainly palmitate and oleate), inhibit the proliferation and promote the differentiation of rapidly dividing keratinocytes, and stimulate the growth of slowly proliferating epidermal cells. To determine the species of PG most effective at modulating keratinocyte proliferation, primary mouse keratinocytes were treated with different PG species, and proliferation was measured. PG species containing polyunsaturated fatty acids were effective at inhibiting rapidly proliferating keratinocytes, whereas PG species with monounsaturated fatty acids were effective at promoting proliferation in slowly dividing cells. Thus, palmitoyl-arachidonyl-PG (16:0/20:4), palmitoyl-linoleoyl-PG (16:0/18:2), dilinoleoyl-PG (18:2/18:2) and soy PG (a PG mixture with a large percentage of polyunsaturated fatty acids) were particularly effective at inhibiting proliferation in rapidly dividing keratinocytes. Conversely, palmitoyl-oleoyl-PG (16:0/18:1) and dioleoyl-PG (18:1/18:1) were especially effective proproliferative PG species. This result represents the first demonstration of opposite effects of different species of a single class of phospholipid and suggests that these different PG species may signal to diverse effector enzymes to differentially affect keratinocyte proliferation and normalize keratinocyte proliferation. Thus, different PG species may be useful for treating skin diseases characterized by excessive or insufficient proliferation.
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U2 - 10.1371/journal.pone.0107119
DO - 10.1371/journal.pone.0107119
M3 - Article
C2 - 25233484
AN - SCOPUS:84907215677
SN - 1932-6203
VL - 9
JO - PloS one
JF - PloS one
IS - 9
M1 - e107119
ER -