Effect of dietary sesame oil as antioxidant on brain hippocampus of rat in focal cerebral ischemia

Saif Ahmad; Seema Yousuf; Tauheed Ishrat; M. Badruzzaman Khan; Kanchan Bhatia; Inayat Salem Fazli; Jafar Salamat Khan; Naseem Hasan Ansari; Fakhrul Islam

doi:10.1016/j.lfs.2006.06.017

Effect of dietary sesame oil as antioxidant on brain hippocampus of rat in focal cerebral ischemia

Saif Ahmad, Seema Yousuf, Tauheed Ishrat, M. Badruzzaman Khan, Kanchan Bhatia, Inayat Salem Fazli, Jafar Salamat Khan, Naseem Hasan Ansari, Fakhrul Islam

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

Oxidative stress may be regarded as an imbalance between free radical production and opposing antioxidant defenses. Free radical oxidative stress is implicated in rat cerebral ischemia and naturaceutical antioxidants are dietary supplements that have been reported to have neuroprotective activity. Many studies have reported dietary sesame oil (SO) as an effective antioxidant. In the present study the neuroprotective effect of dietary SO was evaluated against middle cerebral artery occlusion (MCAO)-induced cerebral ischemia injury in rats. Rats were fed on diet (20% SO) for 15 days. The middle cerebral artery of adult male Wistar rat was occluded for 2 h and reperfused for 22 h. The antioxidant properties of brain were measured as levels of reduced glutathione (GSH), glutathione-S-transferase (GST), glutathione peroxide (GPx), glutathione reductase (GR), catalase (CAT), superoxide dismutase (SOD) and thiobarbituric acid reactive substance (TBARS). A decrease in the activity of all the enzymatic and non-enzymatic antioxidants was observed along with an increase in lipid peroxidation (LPO) in MCAO group. The neurobehavioral activity of rats was also observed by using videopath analyzer. Dietary SO improved the antioxidant status in MCAO + SO group when compared with MCAO group. The results of neurobehavioral activity also support our biochemical data. The results obtained suggest protective effect of SO against cerebral ischemia in rat brain through their antioxidant properties.

Original language	English (US)
Pages (from-to)	1921-1928
Number of pages	8
Journal	Life sciences
Volume	79
Issue number	20
DOIs	https://doi.org/10.1016/j.lfs.2006.06.017
State	Published - Oct 12 2006
Externally published	Yes

Keywords

Antioxidant
Cerebral ischemia
Dietary sesame oil (SO)
Hippocampus
Neurobehavioral activity
Oxidative stress

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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10.1016/j.lfs.2006.06.017

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@article{bbf5b7a418114d4893b04affa7ae7a5a,

title = "Effect of dietary sesame oil as antioxidant on brain hippocampus of rat in focal cerebral ischemia",

abstract = "Oxidative stress may be regarded as an imbalance between free radical production and opposing antioxidant defenses. Free radical oxidative stress is implicated in rat cerebral ischemia and naturaceutical antioxidants are dietary supplements that have been reported to have neuroprotective activity. Many studies have reported dietary sesame oil (SO) as an effective antioxidant. In the present study the neuroprotective effect of dietary SO was evaluated against middle cerebral artery occlusion (MCAO)-induced cerebral ischemia injury in rats. Rats were fed on diet (20% SO) for 15 days. The middle cerebral artery of adult male Wistar rat was occluded for 2 h and reperfused for 22 h. The antioxidant properties of brain were measured as levels of reduced glutathione (GSH), glutathione-S-transferase (GST), glutathione peroxide (GPx), glutathione reductase (GR), catalase (CAT), superoxide dismutase (SOD) and thiobarbituric acid reactive substance (TBARS). A decrease in the activity of all the enzymatic and non-enzymatic antioxidants was observed along with an increase in lipid peroxidation (LPO) in MCAO group. The neurobehavioral activity of rats was also observed by using videopath analyzer. Dietary SO improved the antioxidant status in MCAO + SO group when compared with MCAO group. The results of neurobehavioral activity also support our biochemical data. The results obtained suggest protective effect of SO against cerebral ischemia in rat brain through their antioxidant properties.",

keywords = "Antioxidant, Cerebral ischemia, Dietary sesame oil (SO), Hippocampus, Neurobehavioral activity, Oxidative stress",

author = "Saif Ahmad and Seema Yousuf and Tauheed Ishrat and Khan, {M. Badruzzaman} and Kanchan Bhatia and Fazli, {Inayat Salem} and Khan, {Jafar Salamat} and Ansari, {Naseem Hasan} and Fakhrul Islam",

note = "Funding Information: Dr. Saif Ahmad is thankful to the AYUSH (Ministry of Health and Family Welfare, Government of India, New Delhi) for providing research fellowship. Dr. S. N. Khan (DST, Govt. of India) is gratefully acknowledged for her generous help and valuable suggestions. The authors thank the lab attendants, Mr. Dharamvir and Mr. Anil for the preparation of the diet, and technical assistance. Conflict of Interest Statement: There is no conflict of interest with any person or other organization.",

year = "2006",

month = oct,

day = "12",

doi = "10.1016/j.lfs.2006.06.017",

language = "English (US)",

volume = "79",

pages = "1921--1928",

journal = "Life sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "20",

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TY - JOUR

T1 - Effect of dietary sesame oil as antioxidant on brain hippocampus of rat in focal cerebral ischemia

AU - Ahmad, Saif

AU - Yousuf, Seema

AU - Ishrat, Tauheed

AU - Khan, M. Badruzzaman

AU - Bhatia, Kanchan

AU - Fazli, Inayat Salem

AU - Khan, Jafar Salamat

AU - Ansari, Naseem Hasan

AU - Islam, Fakhrul

N1 - Funding Information: Dr. Saif Ahmad is thankful to the AYUSH (Ministry of Health and Family Welfare, Government of India, New Delhi) for providing research fellowship. Dr. S. N. Khan (DST, Govt. of India) is gratefully acknowledged for her generous help and valuable suggestions. The authors thank the lab attendants, Mr. Dharamvir and Mr. Anil for the preparation of the diet, and technical assistance. Conflict of Interest Statement: There is no conflict of interest with any person or other organization.

PY - 2006/10/12

Y1 - 2006/10/12

N2 - Oxidative stress may be regarded as an imbalance between free radical production and opposing antioxidant defenses. Free radical oxidative stress is implicated in rat cerebral ischemia and naturaceutical antioxidants are dietary supplements that have been reported to have neuroprotective activity. Many studies have reported dietary sesame oil (SO) as an effective antioxidant. In the present study the neuroprotective effect of dietary SO was evaluated against middle cerebral artery occlusion (MCAO)-induced cerebral ischemia injury in rats. Rats were fed on diet (20% SO) for 15 days. The middle cerebral artery of adult male Wistar rat was occluded for 2 h and reperfused for 22 h. The antioxidant properties of brain were measured as levels of reduced glutathione (GSH), glutathione-S-transferase (GST), glutathione peroxide (GPx), glutathione reductase (GR), catalase (CAT), superoxide dismutase (SOD) and thiobarbituric acid reactive substance (TBARS). A decrease in the activity of all the enzymatic and non-enzymatic antioxidants was observed along with an increase in lipid peroxidation (LPO) in MCAO group. The neurobehavioral activity of rats was also observed by using videopath analyzer. Dietary SO improved the antioxidant status in MCAO + SO group when compared with MCAO group. The results of neurobehavioral activity also support our biochemical data. The results obtained suggest protective effect of SO against cerebral ischemia in rat brain through their antioxidant properties.

AB - Oxidative stress may be regarded as an imbalance between free radical production and opposing antioxidant defenses. Free radical oxidative stress is implicated in rat cerebral ischemia and naturaceutical antioxidants are dietary supplements that have been reported to have neuroprotective activity. Many studies have reported dietary sesame oil (SO) as an effective antioxidant. In the present study the neuroprotective effect of dietary SO was evaluated against middle cerebral artery occlusion (MCAO)-induced cerebral ischemia injury in rats. Rats were fed on diet (20% SO) for 15 days. The middle cerebral artery of adult male Wistar rat was occluded for 2 h and reperfused for 22 h. The antioxidant properties of brain were measured as levels of reduced glutathione (GSH), glutathione-S-transferase (GST), glutathione peroxide (GPx), glutathione reductase (GR), catalase (CAT), superoxide dismutase (SOD) and thiobarbituric acid reactive substance (TBARS). A decrease in the activity of all the enzymatic and non-enzymatic antioxidants was observed along with an increase in lipid peroxidation (LPO) in MCAO group. The neurobehavioral activity of rats was also observed by using videopath analyzer. Dietary SO improved the antioxidant status in MCAO + SO group when compared with MCAO group. The results of neurobehavioral activity also support our biochemical data. The results obtained suggest protective effect of SO against cerebral ischemia in rat brain through their antioxidant properties.

KW - Antioxidant

KW - Cerebral ischemia

KW - Dietary sesame oil (SO)

KW - Hippocampus

KW - Neurobehavioral activity

KW - Oxidative stress

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UR - http://www.scopus.com/inward/citedby.url?scp=33749437724&partnerID=8YFLogxK

U2 - 10.1016/j.lfs.2006.06.017

DO - 10.1016/j.lfs.2006.06.017

M3 - Article

C2 - 16822528

AN - SCOPUS:33749437724

SN - 0024-3205

VL - 79

SP - 1921

EP - 1928

JO - Life sciences

JF - Life sciences

IS - 20

ER -

Effect of dietary sesame oil as antioxidant on brain hippocampus of rat in focal cerebral ischemia

Abstract

Keywords

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