Effect of high K+ exposure on phosphoinositide metabolism in frog skeletal muscle

Zheng Dong, Ke Ying Chen, Pei Hong Zhu

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Using [3H]myo-inositol labeled frog skeletal muscles, we have studied the effect of high K+ exposure on phosphoinositide metabolism. After 12 hours labeling, 80mM K+ exposure induced a time-dependent change. The labeling associated with phosphatidylinositol(PI) and phosphatidylinositol 4-phosphate(PIP) gradually increased and decreased, respectively. The labeled phosphatidylinositol 4,5-bisphosphate(PIP2) first decreased, and then recovered. An accumulation of the labeling in inositol phosphates was shown. In shortening the labeling to 30min, 15min high K+ exposure was found to only increase the labeling in all fractions. Taken together, these results show that high K+ exposure can activate the turnover of phosphoinositides, which is consistent with the hypothesis that the metabolism of phosphoiositides may regulate excitation-contraction(e-c) coupling.

Original languageEnglish (US)
Pages (from-to)767-772
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Jan 31 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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