Effect of inoculum size on the induction of endophthalmitis in aphakic rabbit eyes

Robert K. Shockley; Walter M. Jay; Peggy H. Fishman; Mervat Z. Aziz; J. Peter Rissing

doi:10.1111/j.1755-3768.1985.tb05212.x

Effect of inoculum size on the induction of endophthalmitis in aphakic rabbit eyes

Robert K. Shockley, Walter M. Jay, Peggy H. Fishman, Mervat Z. Aziz, J. Peter Rissing

Infectious Disease

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Abstract. A reproducible animal model is necessary to examine the use of antimicrobial agents for prophylaxis and treatment of bacterial endophthalmitis. We determined the minimum inoculum size of S. aureus and P. aeruginosa that consistently produced endophthalmitis when injected into aphakic rabbit eyes immediately following surgery. Both anterior chamber and intravitreal injections were examined. For S. aureus, an intravitreal inoculum of 19.3 ± 7.5 CFU and an anterior chamber inoculum of 50.5 ± 4.0 CFU were required. For P. aeruginosa, an intravitreal inoculum of 5.5 ± 2.6 CFU and an anterior chamber inoculum of 97.5 ± 10.7 CFU consistently produced a fulminant infection. Lower inocula of both bacteria produced endophthalmitis in both locations, but the effect was inconsistent. 1985 Institution Acta Ophthalmologica Scandinavica

Original language	English (US)
Pages (from-to)	35-38
Number of pages	4
Journal	Acta Ophthalmologica
Volume	63
Issue number	1
DOIs	https://doi.org/10.1111/j.1755-3768.1985.tb05212.x
State	Published - Feb 1985

Keywords

P. aeruginosa
S. aureus
aphakia
endophthalmitis
inoculum

ASJC Scopus subject areas

Ophthalmology

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10.1111/j.1755-3768.1985.tb05212.x

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title = "Effect of inoculum size on the induction of endophthalmitis in aphakic rabbit eyes",

abstract = "Abstract. A reproducible animal model is necessary to examine the use of antimicrobial agents for prophylaxis and treatment of bacterial endophthalmitis. We determined the minimum inoculum size of S. aureus and P. aeruginosa that consistently produced endophthalmitis when injected into aphakic rabbit eyes immediately following surgery. Both anterior chamber and intravitreal injections were examined. For S. aureus, an intravitreal inoculum of 19.3 ± 7.5 CFU and an anterior chamber inoculum of 50.5 ± 4.0 CFU were required. For P. aeruginosa, an intravitreal inoculum of 5.5 ± 2.6 CFU and an anterior chamber inoculum of 97.5 ± 10.7 CFU consistently produced a fulminant infection. Lower inocula of both bacteria produced endophthalmitis in both locations, but the effect was inconsistent. 1985 Institution Acta Ophthalmologica Scandinavica",

keywords = "P. aeruginosa, S. aureus, aphakia, endophthalmitis, inoculum",

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AU - Jay, Walter M.

AU - Fishman, Peggy H.

AU - Aziz, Mervat Z.

AU - Rissing, J. Peter

PY - 1985/2

Y1 - 1985/2

N2 - Abstract. A reproducible animal model is necessary to examine the use of antimicrobial agents for prophylaxis and treatment of bacterial endophthalmitis. We determined the minimum inoculum size of S. aureus and P. aeruginosa that consistently produced endophthalmitis when injected into aphakic rabbit eyes immediately following surgery. Both anterior chamber and intravitreal injections were examined. For S. aureus, an intravitreal inoculum of 19.3 ± 7.5 CFU and an anterior chamber inoculum of 50.5 ± 4.0 CFU were required. For P. aeruginosa, an intravitreal inoculum of 5.5 ± 2.6 CFU and an anterior chamber inoculum of 97.5 ± 10.7 CFU consistently produced a fulminant infection. Lower inocula of both bacteria produced endophthalmitis in both locations, but the effect was inconsistent. 1985 Institution Acta Ophthalmologica Scandinavica

AB - Abstract. A reproducible animal model is necessary to examine the use of antimicrobial agents for prophylaxis and treatment of bacterial endophthalmitis. We determined the minimum inoculum size of S. aureus and P. aeruginosa that consistently produced endophthalmitis when injected into aphakic rabbit eyes immediately following surgery. Both anterior chamber and intravitreal injections were examined. For S. aureus, an intravitreal inoculum of 19.3 ± 7.5 CFU and an anterior chamber inoculum of 50.5 ± 4.0 CFU were required. For P. aeruginosa, an intravitreal inoculum of 5.5 ± 2.6 CFU and an anterior chamber inoculum of 97.5 ± 10.7 CFU consistently produced a fulminant infection. Lower inocula of both bacteria produced endophthalmitis in both locations, but the effect was inconsistent. 1985 Institution Acta Ophthalmologica Scandinavica

KW - P. aeruginosa

KW - S. aureus

KW - aphakia

KW - endophthalmitis

KW - inoculum

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Effect of inoculum size on the induction of endophthalmitis in aphakic rabbit eyes

Abstract

Keywords

ASJC Scopus subject areas

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