Abstract
The effect of parathyroid hormone (PTH) and calcitonin on 3H‐choline uptake and on 3H‐acetylcholine synthesis by rat parathyroid glands were examined. Incubation of tissue for 120 min with PTH or calcitonin resulted in an inverted bell‐shaped, dose‐dependent inhibition of 3H‐choline uptake, with a maximal effect at 10−9 M Concentration. The effect of PTH on parathyroid choline uptake was blunted by preincubation with the PTH antagonist NLe8–18 ‐PTH (3–34) amide. PTH brought about a dose‐dependent inhibition of 3H‐choline conversion to 3H‐acetylcholine, with significant effects at 10−8 M concentration or higher. This inhibitory effect of PTH on 3H‐acetylcholine synthesis was blocked by co‐incubation with the PTH antagonist NLe8–18‐PTH (3–34) amide. Only at a 10−7 M concentration calcitonin was effective to impair the in vitro conversion of radioactive choline into 3H‐acetylcholine by parathyroid fragments. The results indicate that, in vitro, PTH, and to a less extent, calcitonin, inhibit cholinergic activity in rat parathyroid glands.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 689-693 |
| Number of pages | 5 |
| Journal | Journal of Neuroendocrinology |
| Volume | 7 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 1995 |
| Externally published | Yes |
Keywords
- acetylcholine
- calcitonin
- parasympathetic nervous system
- parathyroid glands
- parathyroid hormone
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Endocrinology
- Endocrine and Autonomic Systems
- Cellular and Molecular Neuroscience
