Abstract
RT-PCR-based assays for the detection of SARS-CoV-2 have played an essential role in the current COVID-19 pandemic. However, the sample collection and test reagents are in short supply, primarily due to supply chain issues. Thus, to eliminate testing constraints, we have optimized three key process variables: RNA extraction and RT-PCR reactions, different sample types and media to facilitate SARS-CoV-2 testing. By performing various validation and bridging studies, we have shown that various sample types such as nasopharyngeal swab, bronchioalveolar lavage and saliva, collected using conventional nasopharyngeal swabs, ESwab or 3D-printed swabs and, preserved in viral transport media, universal transport media, 0.9% sodium chloride or Amies media are compatible with RT-PCR assay for COVID-19. Besides, the reduction of PCR reagents by up to fourfold also produces reliable results.
Original language | English (US) |
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Pages (from-to) | 1483-1487 |
Number of pages | 5 |
Journal | Future Microbiology |
Volume | 15 |
Issue number | 15 |
DOIs | |
State | Published - Oct 2020 |
Keywords
- 3D swab
- COVID-19
- assay modifications
- assay optimization
- bridging studies
ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)