Effects of maternal medication use on NGF and IL-6 levels in human breast milk

Kashmira Wani; Maya Maliekal; Marilynn R. Fairfax; Rahul Rajkumar; Navya Talluri; Holly Cox; Eunice Woldt; Nitin Shasikant Chouthai

doi:10.1080/14767058.2016.1247152

Effects of maternal medication use on NGF and IL-6 levels in human breast milk

Kashmira Wani, Maya Maliekal, Marilynn R. Fairfax, Rahul Rajkumar, Navya Talluri, Holly Cox, Eunice Woldt, Nitin Shasikant Chouthai

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Background: The objective of this study was to evaluate the effect of maternal medications on nerve growth factor (NGF) and interleukin-6 (IL-6) levels in human breast milk (HBM). Methods: A total of 30 samples of HBM were collected after consent from consecutively born term newborns. NGF and IL-6 concentrations were analyzed using ELISA assays from R&D Systems. The HBM samples were centrifuged, and the clear portion of the HBM after discarding the fat was analyzed and cytokine data were expressed as NGFC or IL-6C. Ten samples of HBM, which were not centrifuged, were also used in ELISA assays and cytokine data were expressed as NGFF or IL-6F. Results: After exposure to NSAIDs (7636 ± 9610, mean ± SD, pg/mL), the NGFC levels in HBM were significantly higher as compared to those who were exposed to narcotics (522 ± 1000) (p = 0.008). NGFC and IL-6C levels positively correlated with each other in HBM (R = 0.194 p < 0.0001). NGFC levels (360 ± 237) were significantly lower than NGFF levels (888 ± 751) (p < 0.0001). IL-6F was higher than IL-6C levels without statistical significance. Conclusion: Further studies are warranted to elucidate effect of maternal medications on cytokine changes in HBM and effect of these cytokine changes on newborn gastrointestinal milieu.

Original language	English (US)
Pages (from-to)	2287-2290
Number of pages	4
Journal	Journal of Maternal-Fetal and Neonatal Medicine
Volume	30
Issue number	19
DOIs	https://doi.org/10.1080/14767058.2016.1247152
State	Published - Oct 2 2017
Externally published	Yes

Keywords

Breastmilk
IL-6
NGF
NSAIDs
cytokines

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Obstetrics and Gynecology

Access to Document

10.1080/14767058.2016.1247152

Cite this

@article{151af31d4b574c83834377cea44ef386,

title = "Effects of maternal medication use on NGF and IL-6 levels in human breast milk",

abstract = "Background: The objective of this study was to evaluate the effect of maternal medications on nerve growth factor (NGF) and interleukin-6 (IL-6) levels in human breast milk (HBM). Methods: A total of 30 samples of HBM were collected after consent from consecutively born term newborns. NGF and IL-6 concentrations were analyzed using ELISA assays from R&D Systems. The HBM samples were centrifuged, and the clear portion of the HBM after discarding the fat was analyzed and cytokine data were expressed as NGFC or IL-6C. Ten samples of HBM, which were not centrifuged, were also used in ELISA assays and cytokine data were expressed as NGFF or IL-6F. Results: After exposure to NSAIDs (7636 ± 9610, mean ± SD, pg/mL), the NGFC levels in HBM were significantly higher as compared to those who were exposed to narcotics (522 ± 1000) (p = 0.008). NGFC and IL-6C levels positively correlated with each other in HBM (R = 0.194 p < 0.0001). NGFC levels (360 ± 237) were significantly lower than NGFF levels (888 ± 751) (p < 0.0001). IL-6F was higher than IL-6C levels without statistical significance. Conclusion: Further studies are warranted to elucidate effect of maternal medications on cytokine changes in HBM and effect of these cytokine changes on newborn gastrointestinal milieu.",

keywords = "Breastmilk, IL-6, NGF, NSAIDs, cytokines",

author = "Kashmira Wani and Maya Maliekal and Fairfax, {Marilynn R.} and Rahul Rajkumar and Navya Talluri and Holly Cox and Eunice Woldt and Chouthai, {Nitin Shasikant}",

note = "Publisher Copyright: {\textcopyright} 2016 Informa UK Limited, trading as Taylor & Francis Group.",

year = "2017",

month = oct,

day = "2",

doi = "10.1080/14767058.2016.1247152",

language = "English (US)",

volume = "30",

pages = "2287--2290",

journal = "Journal of Maternal-Fetal and Neonatal Medicine",

issn = "1476-7058",

publisher = "Informa Healthcare",

number = "19",

}

TY - JOUR

T1 - Effects of maternal medication use on NGF and IL-6 levels in human breast milk

AU - Wani, Kashmira

AU - Maliekal, Maya

AU - Fairfax, Marilynn R.

AU - Rajkumar, Rahul

AU - Talluri, Navya

AU - Cox, Holly

AU - Woldt, Eunice

AU - Chouthai, Nitin Shasikant

PY - 2017/10/2

Y1 - 2017/10/2

N2 - Background: The objective of this study was to evaluate the effect of maternal medications on nerve growth factor (NGF) and interleukin-6 (IL-6) levels in human breast milk (HBM). Methods: A total of 30 samples of HBM were collected after consent from consecutively born term newborns. NGF and IL-6 concentrations were analyzed using ELISA assays from R&D Systems. The HBM samples were centrifuged, and the clear portion of the HBM after discarding the fat was analyzed and cytokine data were expressed as NGFC or IL-6C. Ten samples of HBM, which were not centrifuged, were also used in ELISA assays and cytokine data were expressed as NGFF or IL-6F. Results: After exposure to NSAIDs (7636 ± 9610, mean ± SD, pg/mL), the NGFC levels in HBM were significantly higher as compared to those who were exposed to narcotics (522 ± 1000) (p = 0.008). NGFC and IL-6C levels positively correlated with each other in HBM (R = 0.194 p < 0.0001). NGFC levels (360 ± 237) were significantly lower than NGFF levels (888 ± 751) (p < 0.0001). IL-6F was higher than IL-6C levels without statistical significance. Conclusion: Further studies are warranted to elucidate effect of maternal medications on cytokine changes in HBM and effect of these cytokine changes on newborn gastrointestinal milieu.

AB - Background: The objective of this study was to evaluate the effect of maternal medications on nerve growth factor (NGF) and interleukin-6 (IL-6) levels in human breast milk (HBM). Methods: A total of 30 samples of HBM were collected after consent from consecutively born term newborns. NGF and IL-6 concentrations were analyzed using ELISA assays from R&D Systems. The HBM samples were centrifuged, and the clear portion of the HBM after discarding the fat was analyzed and cytokine data were expressed as NGFC or IL-6C. Ten samples of HBM, which were not centrifuged, were also used in ELISA assays and cytokine data were expressed as NGFF or IL-6F. Results: After exposure to NSAIDs (7636 ± 9610, mean ± SD, pg/mL), the NGFC levels in HBM were significantly higher as compared to those who were exposed to narcotics (522 ± 1000) (p = 0.008). NGFC and IL-6C levels positively correlated with each other in HBM (R = 0.194 p < 0.0001). NGFC levels (360 ± 237) were significantly lower than NGFF levels (888 ± 751) (p < 0.0001). IL-6F was higher than IL-6C levels without statistical significance. Conclusion: Further studies are warranted to elucidate effect of maternal medications on cytokine changes in HBM and effect of these cytokine changes on newborn gastrointestinal milieu.

KW - Breastmilk

KW - IL-6

KW - NGF

KW - NSAIDs

KW - cytokines

UR - http://www.scopus.com/inward/record.url?scp=84995476605&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84995476605&partnerID=8YFLogxK

U2 - 10.1080/14767058.2016.1247152

DO - 10.1080/14767058.2016.1247152

M3 - Article

C2 - 27734743

AN - SCOPUS:84995476605

SN - 1476-7058

VL - 30

SP - 2287

EP - 2290

JO - Journal of Maternal-Fetal and Neonatal Medicine

JF - Journal of Maternal-Fetal and Neonatal Medicine

IS - 19

ER -

Effects of maternal medication use on NGF and IL-6 levels in human breast milk

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this