Emission and polarization spectrometer for biophysical spectroscopy

John Clark Sutherland; George D. Cimino; John T. Lowe

doi:10.1063/1.1134624

Emission and polarization spectrometer for biophysical spectroscopy

John Clark Sutherland, George D. Cimino, John T. Lowe

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

We have constructed a spectrometer which, using well established techniques, measures both fluorescence and circular dichroism (natural and magnetic) as well as several other parameters of interest in biophysical spectroscopy. This combination is feasible since the various measurements, aside from sharing many optical components, can all use a lock-in amplifier to process electronic signals. Although fluorescence and circular dichroism provide greatly different information about the material under investigation, from the standpoint of instrumentation they are very similar. Combining several functions in a single spectrometer has the advantages of economy and flexibility.

Original language	English (US)
Pages (from-to)	358-360
Number of pages	3
Journal	Review of Scientific Instruments
Volume	47
Issue number	3
DOIs	https://doi.org/10.1063/1.1134624
State	Published - 1976
Externally published	Yes

ASJC Scopus subject areas

Instrumentation

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10.1063/1.1134624

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title = "Emission and polarization spectrometer for biophysical spectroscopy",

abstract = "We have constructed a spectrometer which, using well established techniques, measures both fluorescence and circular dichroism (natural and magnetic) as well as several other parameters of interest in biophysical spectroscopy. This combination is feasible since the various measurements, aside from sharing many optical components, can all use a lock-in amplifier to process electronic signals. Although fluorescence and circular dichroism provide greatly different information about the material under investigation, from the standpoint of instrumentation they are very similar. Combining several functions in a single spectrometer has the advantages of economy and flexibility.",

author = "Sutherland, {John Clark} and Cimino, {George D.} and Lowe, {John T.}",

year = "1976",

doi = "10.1063/1.1134624",

language = "English (US)",

volume = "47",

pages = "358--360",

journal = "Review of Scientific Instruments",

issn = "0034-6748",

publisher = "American Institute of Physics Publising LLC",

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T1 - Emission and polarization spectrometer for biophysical spectroscopy

AU - Sutherland, John Clark

AU - Cimino, George D.

AU - Lowe, John T.

PY - 1976

Y1 - 1976

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AB - We have constructed a spectrometer which, using well established techniques, measures both fluorescence and circular dichroism (natural and magnetic) as well as several other parameters of interest in biophysical spectroscopy. This combination is feasible since the various measurements, aside from sharing many optical components, can all use a lock-in amplifier to process electronic signals. Although fluorescence and circular dichroism provide greatly different information about the material under investigation, from the standpoint of instrumentation they are very similar. Combining several functions in a single spectrometer has the advantages of economy and flexibility.

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JO - Review of Scientific Instruments

JF - Review of Scientific Instruments

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ER -

Emission and polarization spectrometer for biophysical spectroscopy

Abstract

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