Abstract
The simple compounded semi-defined Whitten's medium was supplemented with estradiol-17 beta and progesterone to examine whether they influence the nuclear maturation of porcine cumulus-oocyte complexes. Addition of estradiol-17 beta at concentrations of 1.0 microgram/ml and 10.0 micrograms/ml significantly inhibited the nuclear maturation in comparison to the control group. No significant inhibition of estradiol-17 beta was observed at concentration of 0.1 microgram/ml with respect to the control. After addition of synchronous or subsequent (12 h after the incubation with estradiol-17 beta) supplementation of progesterone at concentration of 1 microgram/ml, maturation rates were as high as control. So progesterone overcame the inhibitory effect of estradiol-17 beta.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 157-159 |
| Number of pages | 3 |
| Journal | Berliner und Münchener tierärztliche Wochenschrift |
| Volume | 106 |
| Issue number | 5 |
| State | Published - May 1993 |
| Externally published | Yes |
ASJC Scopus subject areas
- veterinary(all)