Fluoride flux in the rabbit CCD: A pH-dependent event

Alexander J. Rouch; Gary M. Whitford; Harold T. Campbell

doi:10.1038/ki.1992.47

Fluoride flux in the rabbit CCD: A pH-dependent event

Alexander J. Rouch, Gary M. Whitford, Harold T. Campbell

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Abstract

We measured fluoride flux (J_F; pmol ·; min^-1 · mm^-1) in the isolated rabbit cortical collecting duct (CCD) to investigate the determining factors of J_F. The perfusate contained 100 μM fluoride and the bath was fluoride-free. Osmotically-induced lumen-to-bath water flux did not affect J_F. When perfusate pH was reduced from 7.4 to 6.1 and from 6.1 to 5.0, J_F increased from 0.008 ± 0.002 to 0.027 ± 0.007 (P < 0.01) and from 0.018 ± 0.003 to 0.040 ± 0.005 (P < 0.01), respectively. Acetazolamide at 10^-4 M in the bath reduced JF slightly though not statistically. The anion-transport inhibitor, 4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid (DIDS), at 10^-4 M in the perfusate did not affect J_F. Substitution of luminal chloride with gluconate failed to affect J_F in tubules from normal rabbits or from rabbits treated with deoxycorticosterone which stimulates chloride-bicarbonate exchange in the CCD. J_F showed no correlation with transepithelial voltage which ranged from +4 to -104 mV. We conclude that the luminal pH represents the primary determining factor influencing J_F in the rabbit CCD, and fluoride does not use a chloride-mediated or a DIDS-inhibitory transport pathway.

Original language	English (US)
Pages (from-to)	342-349
Number of pages	8
Journal	Kidney International
Volume	41
Issue number	2
DOIs	https://doi.org/10.1038/ki.1992.47
State	Published - Feb 1992
Externally published	Yes

ASJC Scopus subject areas

Nephrology

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10.1038/ki.1992.47

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@article{66b747dad0c5405a8247f9efd511a5fc,

title = "Fluoride flux in the rabbit CCD: A pH-dependent event",

abstract = "We measured fluoride flux (JF; pmol ·; min-1 · mm-1) in the isolated rabbit cortical collecting duct (CCD) to investigate the determining factors of JF. The perfusate contained 100 μM fluoride and the bath was fluoride-free. Osmotically-induced lumen-to-bath water flux did not affect JF. When perfusate pH was reduced from 7.4 to 6.1 and from 6.1 to 5.0, JF increased from 0.008 ± 0.002 to 0.027 ± 0.007 (P < 0.01) and from 0.018 ± 0.003 to 0.040 ± 0.005 (P < 0.01), respectively. Acetazolamide at 10-4 M in the bath reduced JF slightly though not statistically. The anion-transport inhibitor, 4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid (DIDS), at 10-4 M in the perfusate did not affect JF. Substitution of luminal chloride with gluconate failed to affect JF in tubules from normal rabbits or from rabbits treated with deoxycorticosterone which stimulates chloride-bicarbonate exchange in the CCD. JF showed no correlation with transepithelial voltage which ranged from +4 to -104 mV. We conclude that the luminal pH represents the primary determining factor influencing JF in the rabbit CCD, and fluoride does not use a chloride-mediated or a DIDS-inhibitory transport pathway.",

author = "Rouch, {Alexander J.} and Whitford, {Gary M.} and Campbell, {Harold T.}",

note = "Funding Information: Portions of this work have been reported in abstract form: Rouch AJ, Whitford GM, Campbell HT: FASEB J. 2:A1307 1988. This work was supported by USPHS Grant No. DE-06429 from the National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland. We are grateful to Dr. Mark A. Knepper for his keen insight into the planning of this study. We also thank Dr. James A. Schafer for his critical review of this manuscript and for his kindness in allowing some of the experiments to be completed in his laboratory at the Nephrology Research and Training Center, Birmingham, AL. We gratefully ac- knowledge the helpful suggestions from Dr. Jack Ginsburg, Dr. Gary Bond, and Dr. David Pashley.",

year = "1992",

month = feb,

doi = "10.1038/ki.1992.47",

language = "English (US)",

volume = "41",

pages = "342--349",

journal = "Kidney International",

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T1 - Fluoride flux in the rabbit CCD

T2 - A pH-dependent event

AU - Rouch, Alexander J.

AU - Whitford, Gary M.

AU - Campbell, Harold T.

N1 - Funding Information: Portions of this work have been reported in abstract form: Rouch AJ, Whitford GM, Campbell HT: FASEB J. 2:A1307 1988. This work was supported by USPHS Grant No. DE-06429 from the National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland. We are grateful to Dr. Mark A. Knepper for his keen insight into the planning of this study. We also thank Dr. James A. Schafer for his critical review of this manuscript and for his kindness in allowing some of the experiments to be completed in his laboratory at the Nephrology Research and Training Center, Birmingham, AL. We gratefully ac- knowledge the helpful suggestions from Dr. Jack Ginsburg, Dr. Gary Bond, and Dr. David Pashley.

PY - 1992/2

Y1 - 1992/2

N2 - We measured fluoride flux (JF; pmol ·; min-1 · mm-1) in the isolated rabbit cortical collecting duct (CCD) to investigate the determining factors of JF. The perfusate contained 100 μM fluoride and the bath was fluoride-free. Osmotically-induced lumen-to-bath water flux did not affect JF. When perfusate pH was reduced from 7.4 to 6.1 and from 6.1 to 5.0, JF increased from 0.008 ± 0.002 to 0.027 ± 0.007 (P < 0.01) and from 0.018 ± 0.003 to 0.040 ± 0.005 (P < 0.01), respectively. Acetazolamide at 10-4 M in the bath reduced JF slightly though not statistically. The anion-transport inhibitor, 4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid (DIDS), at 10-4 M in the perfusate did not affect JF. Substitution of luminal chloride with gluconate failed to affect JF in tubules from normal rabbits or from rabbits treated with deoxycorticosterone which stimulates chloride-bicarbonate exchange in the CCD. JF showed no correlation with transepithelial voltage which ranged from +4 to -104 mV. We conclude that the luminal pH represents the primary determining factor influencing JF in the rabbit CCD, and fluoride does not use a chloride-mediated or a DIDS-inhibitory transport pathway.

AB - We measured fluoride flux (JF; pmol ·; min-1 · mm-1) in the isolated rabbit cortical collecting duct (CCD) to investigate the determining factors of JF. The perfusate contained 100 μM fluoride and the bath was fluoride-free. Osmotically-induced lumen-to-bath water flux did not affect JF. When perfusate pH was reduced from 7.4 to 6.1 and from 6.1 to 5.0, JF increased from 0.008 ± 0.002 to 0.027 ± 0.007 (P < 0.01) and from 0.018 ± 0.003 to 0.040 ± 0.005 (P < 0.01), respectively. Acetazolamide at 10-4 M in the bath reduced JF slightly though not statistically. The anion-transport inhibitor, 4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid (DIDS), at 10-4 M in the perfusate did not affect JF. Substitution of luminal chloride with gluconate failed to affect JF in tubules from normal rabbits or from rabbits treated with deoxycorticosterone which stimulates chloride-bicarbonate exchange in the CCD. JF showed no correlation with transepithelial voltage which ranged from +4 to -104 mV. We conclude that the luminal pH represents the primary determining factor influencing JF in the rabbit CCD, and fluoride does not use a chloride-mediated or a DIDS-inhibitory transport pathway.

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DO - 10.1038/ki.1992.47

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JO - Kidney International

JF - Kidney International

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Fluoride flux in the rabbit CCD: A pH-dependent event

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