TY - JOUR
T1 - Formation of Proteasome-PA700 Complexes Directly Correlates with Activation of Peptidase Activity
AU - Gogol, E.
AU - Adams, G.
AU - Crotchett, B.
AU - Slaughter, C.
AU - De Martino, G.
N1 - Publisher Copyright:
© 1998 Microscopy Society of America.
PY - 1998/7/1
Y1 - 1998/7/1
N2 - The eukaryotic 20S proteasome (700 kDa) serves as the core complex for the breakdown of most cytosolic proteins by the ubiquitin-dependent pathway. This cylinder-shaped proteolytic assembly (17 nm long, 11 nm diameter) consists of four stacked heptameric rings that help to form a central cavity where degradation occurs. The catalytic sites are located on the cavity surface of the two middle (3 rings while the outer a rings serve as docking sites for various regulatory complexes. PA700 (700 kDa, 20 protein subunits) is the major regulatory complex of the proteasome, forming a "cap"that attaches to either or both proteasome ends. The resulting superassembly structurally resembles the larger, more active 26S proteasome and exhibits both a higher intrinsic peptidase activity as well as a wider range of substrate specificity, including ubiquitin-tagged proteins. A second regulatory complex, the modulator (300 kDa), further stimulates the effects of PA700 at subsaturating concentrations of the activator with no detectable effect on the proteasome in the absence of PA700.
AB - The eukaryotic 20S proteasome (700 kDa) serves as the core complex for the breakdown of most cytosolic proteins by the ubiquitin-dependent pathway. This cylinder-shaped proteolytic assembly (17 nm long, 11 nm diameter) consists of four stacked heptameric rings that help to form a central cavity where degradation occurs. The catalytic sites are located on the cavity surface of the two middle (3 rings while the outer a rings serve as docking sites for various regulatory complexes. PA700 (700 kDa, 20 protein subunits) is the major regulatory complex of the proteasome, forming a "cap"that attaches to either or both proteasome ends. The resulting superassembly structurally resembles the larger, more active 26S proteasome and exhibits both a higher intrinsic peptidase activity as well as a wider range of substrate specificity, including ubiquitin-tagged proteins. A second regulatory complex, the modulator (300 kDa), further stimulates the effects of PA700 at subsaturating concentrations of the activator with no detectable effect on the proteasome in the absence of PA700.
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U2 - 10.1017/S1431927600025034
DO - 10.1017/S1431927600025034
M3 - Article
AN - SCOPUS:85180107570
SN - 1431-9276
VL - 4
SP - 982
EP - 983
JO - Microscopy and Microanalysis
JF - Microscopy and Microanalysis
ER -