TY - JOUR
T1 - Functional and morphological evaluation of canine veins following preservation in different storage media
AU - Cavallari, Nicola
AU - Abebe, Worku
AU - Mingoli, Andrea
AU - Hunter, William J.
AU - Agrawal, Devendra K.
AU - Sapienza, Paolo
AU - Cavallaro, Antonino
AU - Edwards, John D.
N1 - Funding Information:
1 Supported by the Biomedical Research Support Grant, Creighton University School of Medicine, Omaha, Nebraska. 2To whom correspondence and reprint requests should be addressed at via Pastrengo, 55, 62017-Portorecanati (MC), Italy. 3Current address: Department of Pharmacology, School of Medicine, East Carolina University, Greenville, NC.
PY - 1997/3
Y1 - 1997/3
N2 - Injuries of endothelial and smooth muscle cells of autologous vein due to preservation in standard storage media may be responsible for graft failure. The effects of vein preservation with University of Wisconsin solution (UWs) on endothelial and smooth muscle cell function and morphology were compared to the effects of preservation with autologous whole blood (AWB) and normal saline (NS), which are frequently used in cardiovascular surgery. Canine external jugular and common femoral vein segments were preserved in the different solutions at 4°C for 45 min and 24 hr. Rings (4- 5 mm in length) from control and preserved veins were evaluated by isometric tension studies at 37°C and by scanning and transmission electron microscopy. Differences between groups were evaluated by Student's t test or Mann-Whitney U test and by analysis of the variance, and considered to be significant at P < 0.05. Sensitivities to norepinephrine (NE) showed that a 45-min vein storage in AWB (5.7 ± 0.2 μmol/L) but not in NS (5.8 ± 0.2 μmol/L) or UWs (6.5 ± 0.2 μmol/L) had a deleterious effect on function of smooth muscle (P < 0.05) when compared to control veins (6.6 ± 0.2 μmol/L). Maximum contractile responses and sensitivities to NE were significantly altered (P < 0.05) after 24-hr vein storage in AWB (0.09 ± 0.02 g/mm2 and 5.4 ± 0.07 mmol/L) and NS (0.12 ± 0.03 g/mm2 and 5.6 ± 0.08 μmol/L) but not in UWs (0.36 ± 0.06 g/mm2 and 6.4 ± 0.07 μmol/L). With both storage times, acetylcholine-induced endothelium-dependent maximum relaxations and sensitivities were significantly reduced (P < 0.05) in veins stored in AWB and NS, but not in UWs, compared with controls. Similarly, transmission electron microscopy revealed marked neutrophil migration beneath the intimal surface of vessels and extensive separation and desquamation of endothelial cells with exposure of subendothelial structures in veins stored in AWB and NS. The results suggest that UWs is a suitable storage medium when compared to AWB and NS.
AB - Injuries of endothelial and smooth muscle cells of autologous vein due to preservation in standard storage media may be responsible for graft failure. The effects of vein preservation with University of Wisconsin solution (UWs) on endothelial and smooth muscle cell function and morphology were compared to the effects of preservation with autologous whole blood (AWB) and normal saline (NS), which are frequently used in cardiovascular surgery. Canine external jugular and common femoral vein segments were preserved in the different solutions at 4°C for 45 min and 24 hr. Rings (4- 5 mm in length) from control and preserved veins were evaluated by isometric tension studies at 37°C and by scanning and transmission electron microscopy. Differences between groups were evaluated by Student's t test or Mann-Whitney U test and by analysis of the variance, and considered to be significant at P < 0.05. Sensitivities to norepinephrine (NE) showed that a 45-min vein storage in AWB (5.7 ± 0.2 μmol/L) but not in NS (5.8 ± 0.2 μmol/L) or UWs (6.5 ± 0.2 μmol/L) had a deleterious effect on function of smooth muscle (P < 0.05) when compared to control veins (6.6 ± 0.2 μmol/L). Maximum contractile responses and sensitivities to NE were significantly altered (P < 0.05) after 24-hr vein storage in AWB (0.09 ± 0.02 g/mm2 and 5.4 ± 0.07 mmol/L) and NS (0.12 ± 0.03 g/mm2 and 5.6 ± 0.08 μmol/L) but not in UWs (0.36 ± 0.06 g/mm2 and 6.4 ± 0.07 μmol/L). With both storage times, acetylcholine-induced endothelium-dependent maximum relaxations and sensitivities were significantly reduced (P < 0.05) in veins stored in AWB and NS, but not in UWs, compared with controls. Similarly, transmission electron microscopy revealed marked neutrophil migration beneath the intimal surface of vessels and extensive separation and desquamation of endothelial cells with exposure of subendothelial structures in veins stored in AWB and NS. The results suggest that UWs is a suitable storage medium when compared to AWB and NS.
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U2 - 10.1006/jsre.1996.4981
DO - 10.1006/jsre.1996.4981
M3 - Article
C2 - 9184668
AN - SCOPUS:0031105462
SN - 0022-4804
VL - 68
SP - 106
EP - 115
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 2
ER -