Functional differences in human neutrophils isolated pre- and post-prandially

David J. Uhlinger; David N. Burnham; Richard E. Mullins; John R. Kalmar; Christopher W. Cutler; Roland R. Arnold; J. David Lambeth; Alfred H. Merrill

doi:10.1016/0014-5793(91)80933-T

Functional differences in human neutrophils isolated pre- and post-prandially

David J. Uhlinger, David N. Burnham, Richard E. Mullins, John R. Kalmar, Christopher W. Cutler, Roland R. Arnold, J. David Lambeth, Alfred H. Merrill

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Activated polymorphonuclear leukocytes have been associated with neoplasia, atherogenesis and reperfusion injury. Since some of these conditions are also correlated with dietary fat, we examined the functional characteristics of leukocytes isolated from subjects before and after consumption of a lipid-rich meal. There was up to 2-fold greater superoxide generation in response to agonists in leukocytes obtained post-prandially; the maximum increase was observed about 4 h after eating and followed the peak (2-4 h) in serum triglycerides. Neutrophils isolated post-prandially also exhibited impaired chemotaxis and defective bacterial killing, but normal phagocytosis. These findings provide a new variable that should be considered in studies of leukocytes.

Original language	English (US)
Pages (from-to)	28-32
Number of pages	5
Journal	FEBS Letters
Volume	286
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(91)80933-T
State	Published - Jul 29 1991
Externally published	Yes

Keywords

Chemotaxis
Diet
Lipoprotein
Neutrophil
Respiratory burst

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(91)80933-T

Cite this

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title = "Functional differences in human neutrophils isolated pre- and post-prandially",

abstract = "Activated polymorphonuclear leukocytes have been associated with neoplasia, atherogenesis and reperfusion injury. Since some of these conditions are also correlated with dietary fat, we examined the functional characteristics of leukocytes isolated from subjects before and after consumption of a lipid-rich meal. There was up to 2-fold greater superoxide generation in response to agonists in leukocytes obtained post-prandially; the maximum increase was observed about 4 h after eating and followed the peak (2-4 h) in serum triglycerides. Neutrophils isolated post-prandially also exhibited impaired chemotaxis and defective bacterial killing, but normal phagocytosis. These findings provide a new variable that should be considered in studies of leukocytes.",

keywords = "Chemotaxis, Diet, Lipoprotein, Neutrophil, Respiratory burst",

author = "Uhlinger, {David J.} and Burnham, {David N.} and Mullins, {Richard E.} and Kalmar, {John R.} and Cutler, {Christopher W.} and Arnold, {Roland R.} and Lambeth, {J. David} and Merrill, {Alfred H.}",

note = "Funding Information: Acknowledgement: This work was supported by NIH CA46508.",

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doi = "10.1016/0014-5793(91)80933-T",

language = "English (US)",

volume = "286",

pages = "28--32",

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T1 - Functional differences in human neutrophils isolated pre- and post-prandially

AU - Uhlinger, David J.

AU - Burnham, David N.

AU - Mullins, Richard E.

AU - Kalmar, John R.

AU - Cutler, Christopher W.

AU - Arnold, Roland R.

AU - Lambeth, J. David

AU - Merrill, Alfred H.

N1 - Funding Information: Acknowledgement: This work was supported by NIH CA46508.

PY - 1991/7/29

Y1 - 1991/7/29

N2 - Activated polymorphonuclear leukocytes have been associated with neoplasia, atherogenesis and reperfusion injury. Since some of these conditions are also correlated with dietary fat, we examined the functional characteristics of leukocytes isolated from subjects before and after consumption of a lipid-rich meal. There was up to 2-fold greater superoxide generation in response to agonists in leukocytes obtained post-prandially; the maximum increase was observed about 4 h after eating and followed the peak (2-4 h) in serum triglycerides. Neutrophils isolated post-prandially also exhibited impaired chemotaxis and defective bacterial killing, but normal phagocytosis. These findings provide a new variable that should be considered in studies of leukocytes.

AB - Activated polymorphonuclear leukocytes have been associated with neoplasia, atherogenesis and reperfusion injury. Since some of these conditions are also correlated with dietary fat, we examined the functional characteristics of leukocytes isolated from subjects before and after consumption of a lipid-rich meal. There was up to 2-fold greater superoxide generation in response to agonists in leukocytes obtained post-prandially; the maximum increase was observed about 4 h after eating and followed the peak (2-4 h) in serum triglycerides. Neutrophils isolated post-prandially also exhibited impaired chemotaxis and defective bacterial killing, but normal phagocytosis. These findings provide a new variable that should be considered in studies of leukocytes.

KW - Chemotaxis

KW - Diet

KW - Lipoprotein

KW - Neutrophil

KW - Respiratory burst

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ER -

Functional differences in human neutrophils isolated pre- and post-prandially

Abstract

Keywords

ASJC Scopus subject areas

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