TY - JOUR
T1 - Ganglioside GD3 regulates dendritic growth in newborn neurons in adult mouse hippocampus via modulation of mitochondrial dynamics
AU - Tang, Fu Lei
AU - Wang, Jing
AU - Itokazu, Yutaka
AU - Yu, Robert K.
N1 - Funding Information:
This work was supported by an NIH grant (R01 NS100839) and a Sheffield Memorial Award from CSRA Parkinson Support Group to RKY. We thank Dr Yong Li and the Small Animal Behavioral Core, AU, for discussion and help with the behavior analysis.
Publisher Copyright:
© 2020 International Society for Neurochemistry
PY - 2021/3
Y1 - 2021/3
N2 - Ganglioside GD3, a major ganglioside species in neural stem cells, plays a crucial role in maintenance of the self-renewal capacity of these cells. However, its bioactivity in postnatally differentiated neurons in the neurogenic regions of adult brains has not been elucidated. Here, we describe for the first time that deletion of GD3 not only impairs neurotrophin-induced stem cell proliferation, but also alters the dendritic structure as well as the number of synapses of nascent neurons in the dentate gyrus of adult brain. When examining the behavioral phenotypes, GD3 synthase-knockout (GD3S-KO) mice displayed impairment in hippocampus-dependent memory function. To further gain insight into its cellular function, we examined GD3-binding partners from mouse brain extract using a GD3-specific monoclonal antibody, R24, followed by LC-MS/MS analysis and identified a mitochondrial fission protein, the dynamin-related protein-1 (Drp1), as a novel GD3-binding protein. Biochemical and imaging analyses revealed mitochondrial fragmentation in GD3-depleted dentate gyrus neurons, suggesting that GD3 is essential for the mitochondrial Drp1 turnover that is required for efficient mitochondrial fission. These results suggest that GD3 is required for proper dendritic and spine maturation of newborn neurons in adult brain through the regulation of mitochondrial dynamics. (Figure presented.).
AB - Ganglioside GD3, a major ganglioside species in neural stem cells, plays a crucial role in maintenance of the self-renewal capacity of these cells. However, its bioactivity in postnatally differentiated neurons in the neurogenic regions of adult brains has not been elucidated. Here, we describe for the first time that deletion of GD3 not only impairs neurotrophin-induced stem cell proliferation, but also alters the dendritic structure as well as the number of synapses of nascent neurons in the dentate gyrus of adult brain. When examining the behavioral phenotypes, GD3 synthase-knockout (GD3S-KO) mice displayed impairment in hippocampus-dependent memory function. To further gain insight into its cellular function, we examined GD3-binding partners from mouse brain extract using a GD3-specific monoclonal antibody, R24, followed by LC-MS/MS analysis and identified a mitochondrial fission protein, the dynamin-related protein-1 (Drp1), as a novel GD3-binding protein. Biochemical and imaging analyses revealed mitochondrial fragmentation in GD3-depleted dentate gyrus neurons, suggesting that GD3 is essential for the mitochondrial Drp1 turnover that is required for efficient mitochondrial fission. These results suggest that GD3 is required for proper dendritic and spine maturation of newborn neurons in adult brain through the regulation of mitochondrial dynamics. (Figure presented.).
KW - adult neurogenesis
KW - dynamin-related protein 1
KW - ganglioside GD3
KW - mitochondrial dynamics
KW - neuronal morphogenesis
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U2 - 10.1111/jnc.15137
DO - 10.1111/jnc.15137
M3 - Article
C2 - 32743804
AN - SCOPUS:85089387583
SN - 0022-3042
VL - 156
SP - 819
EP - 833
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 6
ER -