TY - JOUR
T1 - GCSF receptor regulates antigen uptake and expression of cytokines and costimulatory molecules in dendritic cells
AU - Geng, Degui
AU - Joshi, Sunil K.
AU - Podolsky, Robert
AU - She, Jin Xiong
N1 - Funding Information:
This work was partly supported by grants from the National Institutes of Health (2RO1HD37800, 2P01 AI-42288, 5U24DK58778, 1R21HD050196, 121DK069878, and JDRF 1-2004-661) to JXS.
PY - 2007/1
Y1 - 2007/1
N2 - RNA interference (RNAi), a process that specifically silences target gene expression, is a powerful technique to modulate cellular functions. In this study, we identified two small interference RNA (siRNA) sequences that can specifically and efficiently silence the expression of the granulocyte colony-stimulating factor receptor (GCSF-R) gene and achieved stable knockdown of GCSF-R using pFIV lentivirus containing the GCSF-R siRNA. GCSF-R knockdown significantly reduces the expression of IL-lα, IL-lβ, IL-6, IL-10, H-2Kb, I-Ab, CD80 and CD86, and increases PDL1 and PDL2 expression, while IL-12p35, TGFβ, TNFα and CD40 expression is unaltered. Furthermore, GCSF-R knockdown significantly changes the endocytosis and micro-pinocytosis abilities as well as surface expression of antigens of DC2.4 cells.
AB - RNA interference (RNAi), a process that specifically silences target gene expression, is a powerful technique to modulate cellular functions. In this study, we identified two small interference RNA (siRNA) sequences that can specifically and efficiently silence the expression of the granulocyte colony-stimulating factor receptor (GCSF-R) gene and achieved stable knockdown of GCSF-R using pFIV lentivirus containing the GCSF-R siRNA. GCSF-R knockdown significantly reduces the expression of IL-lα, IL-lβ, IL-6, IL-10, H-2Kb, I-Ab, CD80 and CD86, and increases PDL1 and PDL2 expression, while IL-12p35, TGFβ, TNFα and CD40 expression is unaltered. Furthermore, GCSF-R knockdown significantly changes the endocytosis and micro-pinocytosis abilities as well as surface expression of antigens of DC2.4 cells.
KW - Antigen presentation
KW - Antigen uptake
KW - Dendritic cells
KW - GCSF
KW - GCSF-R
KW - siRNA
UR - http://www.scopus.com/inward/record.url?scp=33748600398&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748600398&partnerID=8YFLogxK
U2 - 10.1016/j.molimm.2006.02.009
DO - 10.1016/j.molimm.2006.02.009
M3 - Article
C2 - 16574228
AN - SCOPUS:33748600398
SN - 0161-5890
VL - 44
SP - 521
EP - 529
JO - Molecular Immunology
JF - Molecular Immunology
IS - 4
ER -