Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts

Gabriela Racholczyk, Rupali Suhag, Magdalena Mazurek, Suzanne M. Dederscheck, Pandelakis A. Koni

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The International Mouse Knockout Consortium aims to generate a knockout mouse for every single gene on a C57BL/6 background. Our ability to generate such mice is hampered by the poor economics of producing blastocysts to achieve germline transmission of C57BL/6 embryonic stem (ES) cells. We demonstrate superior utility of (C3H x BALB/c)F1 blastocysts compared with BALB/c blastocysts, with blastocyst numbers and germline transmission from subsequent chimeras at a rate 2- to 3-fold higher than that produced with BALB/c blastocysts.

Original languageEnglish (US)
Pages (from-to)413-416
Number of pages4
Issue number3
StatePublished - Mar 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)


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